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Permanent, high stability mounting medium for high definition visualization of IHC.
- Aqueous-based permanent mounting medium designed for fluorogenic labeling and ideal for long-term storage of processed tissue
- High definition visualization for IHC, IF, ISH, FISH
HIGHDEF® IHC fluoromount is for mounting tissue specimens stained with fluorescent dyes. Designed for use in fluorescence, but can be used with other applications.
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Product Details
| Formulation |
Liquid. Mounting medium containing <0.1% sodium azide. |
|---|---|
| Technical Info / Product Notes |
HIGHDEF® IHC fluoromount is an aqueous-based mounting medium for applying coverslips to tissues stained with fluorescent dyes. This product is compatible with FITC, phycoerythrin, phycocyanin, and allophycocyanin. It can also be used with other fluorescent markers such as Texas Red and rhodamine. |
Handling & Storage
| Long Term Storage |
Ambient |
|---|---|
| Shipping |
Ambient Temperature |
| Regulatory Status |
RUO – Research Use Only |
|---|
- PD-1-positive cells contribute to the diagnosis of inflammatory bowel disease and can aid in predicting response to vedolizumab: Kim, M. K., Jo, S. I., et al.; Research Square , (2023), Application(s): IHC / Reactant(s): Human
- The Expression of the Alpha7 Nicotinic Acetylcholine Receptor and the Effect of Smoking in Curdlan-Administered SKG Mice.: Kim, Y. E., Lee, J. H., et al.; Biomedicines 11, (2023), Application(s): IHC-IF / Reactant(s): Mouse, Abstract
- PD-1-positive cells contribute to the diagnosis of inflammatory bowel disease and can aid in predicting response to vedolizumab.: Kim, M. K., Jo, S. I., et al.; Sci. Rep. 13, 21329 (2023), Application(s): IHC / Reactant(s): Human, Abstract
- Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx: Kang, T. G., Kwon, K. W., et al.; Nat. Commun. 13, 3155 (2022), Abstract
- Snail acetylation by autophagy-derived acetyl-coenzyme A promotes invasion and metastasis of KRAS-LKB1 co-mutated lung cancer cells: Han, J. H., Kim, Y. K., et al.; Cancer Commun. (Lond.) 42, 716 (2022), Abstract
- Immune profile by multiplexed immunohistochemistry associated with recurrence after chemoradiation in rectal cancer.: Hong, S. W., Lee, S., et al.; J. Gastroenterol. Hepatol. 37, 542 (2022), Reactant(s): Human, Abstract
- Soluble CD163 is produced by monocyte-derived and alveolar macrophages, and is not associated with the severity of idiopathic pulmonary fibrosis: P. Chauvin, et al.; Innate Immun. 28, 138 (2022), Abstract
- The human dental apical papilla promotes spinal cord repair through a paracrine mechanism: P. De Berdt, et al.; Cell. Mol. Life Sci. 79, 252 (2022), Abstract
- T-cell mediated targeted delivery of anti-PD-L1 nanobody overcomes poor antibody penetration and improves PD-L1 blocking at the tumor site: P.F. Petit, et al.; Cancer Immunol. Res. 1158, 2326 (2022), Abstract
- Multiplex Immunofluorescence Assay of Infiltrating Mononu-Clear Cell Subsets in Acute T-Cell-Mediated Rejection and BK Virus-Associated Nephropathy in the Allograft Kidney.: Kim, S. Y., Kim, Y. J., et al.; Diagnostics (Basel) 12, (2022), Reactant(s): Human, Abstract
- Discovery of acquired molecular signature on immune checkpoint inhibitors in paired tumor tissues: Yoo, S. H., Yun, J., et al.; Cancer Immunol. Immunother. 70, 1755 (2021), Abstract
- Dysregulation of TFH-B-TRM lymphocyte cooperation is associated with unfavorable anti-PD-1 responses in EGFR-mutant lung cancer.: Bae, Y. S., Shim, H. S., et al.; Nat. Commun. 12, 6068 (2021), Reactant(s): Human, Abstract
- Acetylated Diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol in Autoimmune Arthritis and Interstitial Lung Disease in SKG Mice.: Lee, J. H., Yoo, B., et al.; Biomedicines 9, (2021), Reactant(s): Mouse, Abstract
- siRNA Nanoparticle Targeting PD-L1 Activates Tumor Immunity and Abrogates Pancreatic Cancer Growth in Humanized Preclinical Model: J.Y. Jung, et al.; Cells 10, 2734 (2021), Abstract
- Dodecanol, metabolite of entomopathogenic fungus Conidiobolus coronatus, affects fatty acid composition and cellular immunity of Galleria mellonella and Calliphora vicina: M. Kazek, et al.; Sci. Rep. 11, 15963 (2021), Abstract
- Increased expression of macrophages and inflammatory cytokines at tendons origin in patients with chronic lateral epicondylitis: B.A.A. Al-Daher, et al.; J. Shoulder Elbow Surg. 30, 1487 (2021), Abstract
- Immune profiling of advanced thyroid cancers using fluorescent multiplex immunohistochemistry: J. Ahn, et al.; Thyroid 31, 61 (2021), Abstract
- Preparation of human decellularized peripheral nerve allograft using amphoteric detergent and nuclease: J. Bae, et al.; Neural Regen. Res. 9, 1890 (2021), Abstract
- Multiplex Immunofluorescence Analysis of Infiltrating Mononuclear Cell Subsets in Acute T-cell-mediated Rejection and Bk Virus-Associated Nephropathy in The Allograft Kidney: Kim, M. S., Kim, Y. J., et al.; Research Square , (2021), Reactant(s): Human
- Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 pulmonary influx: Shin, S. J., Kang, T. G., et al.; Research Square , (2021)
- Integrated pharmaco-proteogenomics defines two subgroups in isocitrate dehydrogenase wild-type glioblastoma with prognostic and therapeutic opportunities: S. Oh, et al.; Nat. Commun. 11, 3288 (2020), Application(s): IHC, Abstract — Full Text
- Long-term advantages of ovarian reserve maintenance and follicle development using adipose tissue-derived stem cells in ovarian tissue transplantation: L. Cacciottola, et al.; J. Clin. Med. 9, 2980 (2020), Abstract — Full Text
- Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis: J. Heo, et al.; EMBO Mol. Med. 12, e10880 (2020), Abstract — Full Text
- Tryptophan 2, 3-dioxygenase expression identified in murine decidual stromal cells is not essential for feto-maternal tolerance: D. Hoffmann, et al.; Front. Immunol. 11, 601759 (2020), Abstract — Full Text
- Human dental stem cells of the apical papilla associated to BDNF-loaded pharmacologically active microcarriers (PAMs) enhance locomotor function after spinal cord: S. Kandalam, et al.; Int. J. Pharm. 587, 119685 (2020), Abstract
- Distinct tumor immune microenvironments in primary and metastatic lesions in gastric cancer patients: S. Son, et al.; Sci. Rep. 10, 14293 (2020), Reactant(s) Human, Abstract — Full Text
- Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer: S. Kim, et al.; J. Gastric Cancer 20, 408 (2020), Reactant(s) Human, Abstract
- Clinical Significance of CLDN18.2 Expression in Diffuse-Type Metastatic Gastric Cancer: Kim, S., Shin, K., et al.; Research Square , (2020)
- Engineering and Characterization of Oncolytic Vaccinia Virus Expressing Truncated Herpes Simplex Virus Thymidine Kinase: Islam, S. M. B. U., Lee, B., et al.; Cancers (Basel) 12, (2020), Abstract
- Immunoprofiling of Colitis-associated and Sporadic Colorectal Cancer and its Clinical Significance: J.S. Soh, et al.; Sci. Rep. 9, 6833 (2019), Reactant(s) Human, Abstract — Full Text
- Preferential Infiltration of Unique Vγ9Jγ2-Vδ2 T Cells Into Glioblastoma Multiforme.: Lee, M., Park, C., et al.; Front. Immunol. 10, 555 (2019), Reactant(s): Human, Abstract
- Structural principles in Robo activation and auto-inhibition: R. Barak, et al.; Cell 177, 272 (2019), Abstract
- Association between the exposure to anti-angiogenic agents and tumour immune microenvironment in advanced gastrointestinal stromal tumours: C. Yoo, et al.; Br. J. Cancer 121, 819 (2019), Abstract — Full Text
- CD56 + CD57 + Infiltrates as the Most Predominant Subset of Intragraft Natural Killer Cells in Renal Transplant Biopsies With Antibody-Mediated Rejection: H.R. Jung, et al.; Sci. Rep. 9, 16606 (2019), Reactant(s) Human, Abstract
- Paired whole exome and transcriptome analyses for the Immunogenomic changes during concurrent chemoradiotherapy in esophageal squamous cell: S. Park, et al.; J. Immunother. Cancer 7, 128 (2019), Application(s): IHC / Reactant(s) Human, Abstract — Full Text
- HADC8 inhibitor WK2-16 therapeutically targets lipopolysaccharide-induced mouse model of neuroinflammation and microglial activation: F.L. Lin, et al.; Int. J. Mol. Sci. 20, 410 (2019), Abstract — Full Text
- Theissenolactone C exhibited ocular protection of endotoxin-induced uveitis by attenuating ocular inflammatory responses and glial activation: F.L. Lin, et al.; Front. Pharmacol. 9, 326 (2018), Abstract — Full Text
- Poor prognosis in Epstein-Barr virus-negative gastric cancer with lymphoid stroma is associated with immune phenotype: C.J. Cho, et al.; Gastric Cancer 21, 925 (2018), Abstract
- In vivo characterization of metabolic activity and oxidative stress in grafted human ovarian tissue using microdialysis: L. Cacciottola, et al.; Fertil. Steril. 110, 534 (2018), Abstract
- Evaluation of lauroyl-gemcitabine-loaded hydrogel efficacy in glioblastoma rat models: C. Bastiancich, et al.; Nanomedicine (Lond.) 13, 1999 (2018), Abstract
- Prediction of radio-responsiveness with immune-profiling in patients with rectal cancer: I.J. Park, et al.; Oncotarget 8, 79793 (2017), Application(s): IHC-IF / Reactant(s) Human, Abstract — Full Text
- The natural retinoprotectant chrysophanol attenuated photoreceptor cell apoptosis in an N-methyl-N-nitrosourea-induced mouse model of retinal degenaration: F.L. Lin, et al.; Sci. Rep. 7, 41086 (2017), Abstract — Full Text
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HIGHDEF® IHC mount
ADI-950-261
Permanent, high stability mounting medium for high definition visualization of IHC.
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Specific Protocol
Mounting Procedure:
- Remove the red tip from the bottle and cut the tip off with a sharp razor or scissors. This will allow smooth flow of HIGHDEF® IHC fluoromount and will prevent formation of bubbles.
- Place the bottle upside down in a container. This will help clear HIGHDEF® IHC fluoromount of tiny bubbles.
- Blot excess water from the slide without letting tissue specimens dry. Tissue should be wet prior to mounting.
- Apply 2-3 drops of HIGHDEF® IHC fluoromount on the tissue section and gently apply a cover slip. Allow HIGHDEF® IHC fluoromount to dry for 30-45 minutes at room temperature. For long term storage, seal coverslip edges with nail polish
Removal of Fluoromount: HIGHDEF® IHC fluoromount is an aqueous-based mounting medium and can easily be removed by soaking slides in deionized water. Place a slide in a beaker full of deionized water on a magnetic stirrer. Allow the slide to soak for few hours (or overnight) with gentle stirring for complete removal of dry HIGHDEF® IHC fluoromount.
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SDS
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