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- Strong, clear color development
- Compatible with high-throughput systems
The HIGHDEF® DAB Chromogen/Substrate Set can be used in conjunction with peroxidase-based immunostaining systems. Peroxidase from the antibody detection system reacts with hydrogen peroxide substrate to degrade it, which then reacts with DAB, precipitating it at positive sites and yielding a dark brown color.
3,3′-Diaminobenzidine (DAB), a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and decreased background when compared to amino ethyl carbazole (AEC). Specimens stained with DAB can be dehydrated, cleared, and mounted for permanent record keeping. HIGHDEF® DAB Chromogen/Substrate Set is more sensitive and stable than traditional working DAB solutions.
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Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from transgenic mouse brain expressing human mutated APP and PS1. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from human brain with Alzheimer’s disease. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
Cancerous tonsil tissue was incubated with an anti-Ki-67 primary antibody and then stained with POLYVIEW® PLUS HRP (anti-Rabbit) Reagent (Cat. No. ENZ-ACC104) and then color was developed using HIGHDEF® DAB Chromogen/Substrate Set.
Cancerous tonsil tissue was incubated with an anti-Ki-67 primary antibody and then stained with POLYVIEW® PLUS HRP (anti-Rabbit) Reagent (Cat. No. ENZ-ACC104) and then color was developed using HIGHDEF® DAB Chromogen/Substrate Set.
| Regulatory Status |
RUO – Research Use Only |
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