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HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridization systems. With at least 1 day working stability, this substrate/chromogen system can be used in any automation system as well as manual use.
Application Note
Tumor Phenotyping and Immune Cell Regulation using Multiplex IHC
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Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight cytokeratin antibody labeled and HIGHDEF® black IHC chromogen (HRP) (Prod. no. ADI-950-171) produces a sharply contrasting ebony black color.
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with CD8 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD68 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD163 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle – PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.
Multiplex IHC staining performed on formalin-fixed paraffin-embedded human tonsil stained with FOXP3 antibody labeled with HIGHDEF® black IHC chromogen (HRP) (Prod. Ref. ADI-950-171), CD3 antibody labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. Ref. ADI-950-170), CD8 antibody labeled with HIGHDEF® green AP chromogen/substrate (Prod. Ref. ENZ-ACC130), and hematoxylin counterstain. (Image courtesy from Plateforme de Pathologie Expérimentale et Translationnelle – PETRA, Unité AMMICa, Gustave Roussy Cancer Campus, Villejuif, France.)
| Regulatory Status |
RUO – Research Use Only |
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