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Hepsin (human), (recombinant)

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Alternative Name	Transmembrane protease serine 1, TMPRSS1
Biological Activity	Trypsin-like serine protease.
Concentration	100nM
Formulation	Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride and 10% glycerol.
MW	~45kDa; a-subunit: ~15kDa; b-subunit: ~30kDa
Source	Produced in Drosophila Schneider 2 (S2) cells. Recombinant human hepsin (aa 45-417) is fused at the C-terminus to a V5-His-tag. Produced by plasmid transfection expression.
Specific Activity	~2.5U/µl. One unit is defined at the amount of enzyme that releases 1nmol of AMC per min. at 37°C in the presence of 50µM Boc-Gln-Ala-Arg-AMC.
UniProt ID	P05981

Use/Stability	Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: 100mM TRIS, pH 9.0, containing 5mg/ml BSA. Do not store diluted solutions.
Handling	Avoid freeze/thaw cycles.
Short Term Storage	-20°C
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J. 276, 2213 (2009), Abstract

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