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Guanylyl cyclase (soluble) (bovine lung)

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Alternative Name	GTP pyrophosphate-lyase (cycling)
Concentration
Formulation	Liquid. In ~20µl containing 50% glycerol, 88mM NaCl, 1mM DTT, 0.5mM EDTA, 25mM triethanolamine at pH 7.4.
Source	Isolated from bovine lung by an immunoaffinity method.
Specific Activity	>10’000nmol cGMP/mg/min at 37°C under NO-stimulated conditions.

Use/Stability	As indicated on product label or CoA when stored as recommended. Product should be active for at least one year after shipping date when stored at -80°C under nitrogen in the presence of 50% glycerol. Dilutions of the enzyme should be peformed in the presence of 0.5mg/ml BSA. The dissolved product is stable for 8 months when stored in 50% glycerol at -80°C.
Handling	Avoid freeze/thaw cycles.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Detection of nitric oxide production in cell cultures by luciferin-luciferase chemiluminescence: Y. Woldman, et al.; Biochem. Biophys. Res. Commun. 465, 232 (2015), Application(s): Cell culture, Abstract
A new small molecule inhibitor of soluble guanylate cyclase: F. Mota, et al.; Bioorg. Med. Chem. 23, 5303 (2015), Abstract — Full Text
Direct chemiluminescence detection of nitric oxide in aqueous solutions using the natural nitric oxide target soluble guanylyl cyclase: Y.Y. Woldman, et al.; Free Radic. Biol. Med. 47, 1339 (2009), Abstract — Full Text
Purification of soluble guanylyl cyclase from bovine lung by a new immunoaffinity chromatographic method: P. Humbert, et al.; Eur. J. Biochem. 190, 273 (1990), Abstract

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