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GroES (E. coli) polyclonal antibody

ADI-SPA-210

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GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.

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This antibody is covered by our Worry-Free Guarantee.

GroES (E. coli) polyclonal antibody Western blot — Western blot analysis of GroES: Lane 1: MW marker, Lane 2: GroES Recombinant E. coli Protein, Lane 3: (Empty), Lane 4: E. coli Lysate.

Product Details

Alternative Name	Chaperonin 10
Application	WB
Application Notes	Detects a band of ~10kDa by Western blot.
Formulation	Liquid. Whole rabbit serum.
GenBank ID	X07850
Host	Rabbit
Immunogen	E. coli GroES.
Recommendation Dilutions/Conditions	Western Blot (1:5,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Whole rabbit serum.
Species Reactivity	E. coli
UniProt ID	P0A6F9 (strain K12)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

GroEL/ES chaperonin unfolds then encapsulates a nascent protein on the ribosome: Roeselová, A., Maslen, S. L., et al.; bioRxiv , (2024), Application(s): WB
The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli.: Valdez-Cruz, N. A., Perez, N. O., et al.; Appl. Microbiol. Biotechnol. 106, 2883 (2022), Application(s): WB / Reactant(s): E. coli, Abstract
Prolyl isomerases in a minimal cell – catalysis of protein folding by trigger factor from Mycoplasma genitalium: A. Pahl, et al.; Eur. J. Biochem. 267, 3270 (2000), Application(s): WB using bacteria (E. coli) samples, Abstract
Expression of a gene encoding a 16.9-kDa heat-shock protein, Oshsp16.9, in Escherichia coli enhances thermotolerance: C.Y. Lin, et al.; PNAS 94, 10967 (1997), Application(s): WB using bacteria (E. coli) samples, Abstract

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GroES (E. coli) polyclonal antibody

ADI-SPA-210

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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