GOLGI ID® Green assay kit

• Novel Golgi apparatus-selective dye stains live or aldehyde-fixed cells
• Fluorescence emission is easily multiplexed with common fluorescent dyes and fluorescent proteins
• Highly resistant to photo-bleaching, concentration quenching and photo-conversion
• Stringently manufactured to control and eliminate non-specific assay artifacts

The GOLGI ID^® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. Compared with other commercially available dyes for labeling Golgi bodies, GOLGI ID^® Green dye is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID^® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. One important application of GOLGI ID^® Green dye is in fluorescence co-localization imaging with red fluorescent protein (RFP)-tagged proteins. This is a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions. Many organelle-targeting probes photo-bleach rapidly, are subject to quenching upon concentration in organelles, are highly toxic or only transiently associate with the target organelle, requiring imaging within a minute or two of dye addition. Consequently, GOLGI ID^® Green dye, a new green-emitting, cell-permeable small molecule organic probe that spontaneously localizes to live or fixed Golgi apparatus, was developed. GOLGI ID^® Green dye can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multicolor imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photo-conversion. The kit should also be suitable for identifying Golgi body perturbing agents and thus can be a useful tool for examining retrograde flow mechanisms in cellular secretory pathways. The GOLGI ID^® Green assay kit has been specifically designed for use with RFP-expressing cell lines as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs). Additionally, the kit is suitable for use with live or fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A blue nuclear counterstain, Hoechst 33342, is provided to highlight this organelle as well.