Home Shop Goat anti-mouse IgM, polyclonal antibody (HRP conjugate)

Goat anti-mouse IgM, polyclonal antibody (HRP conjugate)

ADI-SAB-110

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Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection.

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This antibody is covered by our Worry-Free Guarantee.

Product Details

Application	ELISA, IHC, WB
Crossreactivity	May cross-react with IgM from other species.
Formulation	Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/mL BSA, and 50% glycerol.
Host	Goat
Immunogen	Purified mouse IgM.
Purity Detail	Immunoaffinity purified.
Quality Control	The goat antiserum was immunoaffinity purified to isolate anti-mouse IgM μ-chain specific antibodies which were then adsorbed with human IgG and conjugated to horseradish peroxidase (HRP).
Recommendation Dilutions/Conditions	ELISA (1:2,500-50,000)Immunohistochemistry (1:250-2500)Western Blot (1:2500-50,000 colorimetric, 1:5,000-100,000 ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Mouse
Specificity	Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

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The amplification of CNS damage in Alzheimer’s disease due to SARS-CoV2 infection: G.J. Nuovo, et al.; Ann. Diagn. Pathol. 61, 152057 (2022), Abstract
PTEN-Induced Putative Kinase 1 Dysfunction Accelerates Synucleinopathy: T.T. Nguyen, et al.; J. Parkinsons Dis. 12, 1201 (2022), Abstract
Increased clearance of non-biodegradable polystyrene nanoplastics by exocytosis through inhibition of retrograde intracellular transport: S.W. Han, et al.; J. Hazard. Mater. 439, 129576 (2022), Abstract
A Critical YAP in Malignancy of HCC Is Regulated by Evodiamine: U.J. Yun, et al.; Int. J. Mol. Sci. 23, 1855 (2022), Abstract
Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1: J. Seo, et al.; Int. J. Mol. Sci. 22, 1700 (2021), Abstract — Full Text
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Inhibitory Effect of Elaeagnus umbellata Fractions on Melanogenesis in α-MSH-Stimulated B16-F10 Melanoma Cells: J.H. Lee, et al.; Molecules 26, 1308 (2021), Abstract — Full Text
The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome: M.C. Trotta, et al.; PLoS One 14, e0211005 (2019), Abstract — Full Text