Glycerol mounting medium (100 ml)

This mounting medium is made with glycerol. It is excellent for preserving thick sections that can not be mounted with mounting medium or fresh frozen tissues that contain a lot of lipids e.g. brain tissue. It is also useful for preserving small embryos.
This mounting medium is also useful for immunofluorescence. This is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy dyes, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine and Redox. The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes.
Not recommended for phycoethyrin (PE), phycocyanin (PC) and allophycocyanin (APC).