Home Shop Glutamate decarboxylase (65kDa isoform) (human) monoclonal antibody (83/2E6)

Glutamate decarboxylase (65kDa isoform) (human) monoclonal antibody (83/2E6)

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Alternative Name	GAD65, GAD2
Application	ICC, IHC (FS), IP, Radioimmunoassay
Application Notes	GAD-binding of this antibody is competitively inhibited by human GAD-antibodies.
Clone	83/2E6
Crossreactivity	Does not cross-react with GAD67.
Formulation	Liquid. In PBS. pH 7.4, containing 0.9mg/ml sodium azide.
Host	Mouse
Immunogen	Recombinant human GAD65 (glutamate decarboxylase (65kDa isoform)).
Isotype	IgG1
Purity Detail	Ammonium sulphate precipitation.
Species Reactivity	Human, Rat
Specificity	Recognizes an epitope (aa 221-442) of human GAD65. Binds to human and rat pancreas tissue but not mouse pancreas tissue. Not detectable on Bouin-fixed pancreatic tissues. No binding to viable rat islet cells.
UniProt ID	Q05329
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Use/Stability	Stable for at least 1 year when stored at +4°C.
Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

A monoclonal antibody-based characterization of autoantibodies against glutamic acid decarboxylase in adults with latent autoimmune diabetes: B. Ziegler, et al.; Autoimmunity 28, 61 (1998), Abstract
Immunohistochemical differentiation of monoclonal GAD antibodies recognizing linear or conformational epitope regions: P. Augstein, et al.; Pancreas 15, 139 (1997), Abstract
Comparison of the islet cell antibody pattern of monoclonal glutamic acid decarboxylase antibodies recognizing linear and conformational epitopes: P. Augstein, et al.; Acta Histochem. 98, 229 (1996), Abstract
Monoclonal antibodies specific to the glutamic acid decarboxylase 65 kDa isoform derived from a non-obese diabetic (NOD) mouse: B. Ziegler, et al.; Diabetes Res. 25, 47 (1994), Abstract

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