Global DNA Methylation ELISA Kit (5’-methyl-2’-deoxycytidine Quantitation)

• For detection and quantitation of 5′-methyl-2′-deoxycytidine (5MedCyd)
• Suitable for use with DNA samples such as cell or tissue genomic DNA
• Detection sensitivity of 150 nM 5MedCyd

The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.