AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
- Monitor calcium mobilization in GFP-expressing cell lines
- The brightest and most sensitive red-shifted fluorescent Ca2+ indicator
- Provides comparable EC50 and IC50 values as obtained using Rhod-4 AM and Fluo-4 AM
- Larger assay window and higher Z’ factor values, allowing measurements of challenging cell lines and receptors
Enzo Life Sciences’ GFP-CERTIFIED® FLUOFORTE® Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-CERTIFIED® FLUOFORTE® dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics.
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
Histamine H1 receptors: HeLa cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom microplate. The cells were incubated with 100 µL of GFP-CERTIFIED® FLUOFORTE® reagent or Rhod-2 AM dye for 1 hour at 37°C. Histamine (20µL/well) was added using a BioTek two-syringe pump dispenser to achieve the final indicated concentrations. No significant difference in EC50 of Histamine for GFP-CERTIFIED® FLUOFORTE® and Rhod2-AM dye was observed. GFP-CERTIFIED® FLUOFORTE® reagent generated higher intensity signal and a larger assay window.
U-2 OS cells were incubated with (A) GFP-CERTIFIED® FLUOFORTE® AM dye or (B) Rhod-2 AM dye. Cells were washed and treated with a final concentration of 100nM ATP and imaged immediately. GFP-CERTIFIED® FLUOFORTE® AM dye exhibits brighter fluorescence intensity than Rhod-2 AM.
GFP-CERTIFIED® FLUOFORTE® Calcium assay kit image
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
FLUOFORTE® Calcium assay kit
ENZ-51016
A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
| Application | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
|---|
FLUOFORTE® Calcium assay kit
ENZ-51017
A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
| Application | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
|---|
| Appearance | Lyophilized, orange. |
|---|---|
| Purity | ≥95% (HPLC) |