GAPDH Activity Assay

GAPDH catalyzes the conversion of glyceraldehyde-3-phosphate to 1, 3-bisphosphoglycerate (1,3-BPG), creating a high-energy phosphate bond while simultaneously facilitating the formation of NADH. This is a key step in glycolysis. 1,3-BPG goes on to result in ATP production while the NADH powers the electron transport chain, synthesizing more ATP. GAPDH is an essential constitutive enzyme whose activity is regulated by several factors, including cellular energy demand, redox state and post-translational modifications. Dysregulation of GAPDH is associated with cancer and neurodegenerative disorders. AkrivisBio’s GAPDH Assay is a simple, sensitive means of measuring GAPDH activity in a variety of samples with a sensitivity into the low µU/ml.