FURA-2 (ultra pure)

Fura-2 is one of the most widely used calcium indicators for homogenous quantitative dual wavelength ratiometric cell measurements. Fura-2 is particularly useful for digital imaging microscopy. It is less susceptible to photobleaching than Indo-1. The probe is excited only by UV light, which results in significantly less interference by visible wavelength excitable fluorescent compounds. One application of the probe is as a useful counter screen tool for GPCR and calcium channels identified using calcium indicators excited at 488nm in primary screens. Fura-2 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Wavelength Maxima: Excitation 363nm, Emission 512nm