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FURA-2 AM (ultra pure)

ENZ-52006

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Calcium dye

Fura-2 acetomethoxy (AM) is one of the most widely used calcium indicators for homogenous quantitative dual wavelength ratiometric cell measurements. Fura-2 AM is particularly useful for digital imaging microscopy. It is less susceptible to photobleaching than Indo-1. The probe is excited only by UV light, which results in significantly less interference by visible wavelength excitable fluorescent compounds. One application of the probe is as a useful counter screen tool for GPCR and calcium channel hits identified using calcium indicators excited at 488nm in primary screens. Fura-2 AM can be easily loaded into cells by passive incubation. Wavelength Maxima: Excitation 370nm, Emission 476nm

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Product Details

Alternative Name	1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2′-amino-5′-methyl-phenoxy)ethane-N,N,N’,N’-tetraacetic acid, pentaacetoxymethyl ester
Appearance	Off-white to yellow solid.
CAS	108964-32-5
Formula	C₄₄H₄₇N₃O₂₄
MW	1001.9
Purity	≥95% (HPLC)
Solubility	Soluble in DMSO.
Technical Info / Product Notes	This product is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Handling & Storage

Use/Stability	Stable for at least one year after receipt when stored as recommended.
Handling	Protect from light. Keep cool and dry.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases: Awad, N., Weidinger, D., et al.; Cell Biol. Toxicol. 40, 96 (2024), Abstract
TRPM3 Is Expressed in Afferent Bladder Neurons and Is Upregulated during Bladder Inflammation: M. Vanneste, et al.; Int. J. Mol. Sci. 23, 107 (2022), Abstract
OR2AT4 and OR1A2 counterregulate molecular pathophysiological processes of steroid-resistant inflammatory lung diseases in human alveolar macrophages: Weidinger, D., Jamal Jameel, K., et al.; Mol. Med. 28, 150 (2022), Abstract
Gain of channel function and modified gating properties in TRPM3 mutants causing intellectual disability and epilepsy.: Van Hoeymissen, E., Held, K., et al.; Elife 9, (2020), Reactant(s): Human, Abstract
Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch: Mishra, S. K., Wheeler, J. J., et al.; Cell Rep. 31, 107472 (2020), Abstract
Itch-associated Neuropeptides and Their Receptor Expression in Dog Dorsal Root Ganglia and Spinal Cord.: Olivry, T., Mishra, S. K., et al.; Acta Derm. Venereol. 99, 1131 (2019), Reactant(s): Dog, Abstract
Measurement of [Ca2+]i in whole cell suspensions using fura-2: R.A. Hirst, et al.; Methods Mol. Biol. 312, 37 (2006), Abstract
Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source: G. McConnell, et al.; Opt. Lett. 28, 1742 (2003), Abstract

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FURA-2 AM (ultra pure)

ENZ-52006

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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