Fluoroshield with DAPI & PPD (100 ml)

Fluoroshield with DAPI and PPD is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Fluorescence is retained during prolonged storage at 4°C in the dark.
This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
This medium contains phenylenediamine and is not suitable for immunofluorescence of Cy dyes, GFP, mCherry, Phycoerythrin (R-PE), phyocyanin (PC), allophycocyanin (APC) and other fluorochromes proteins.