Fluorescent Transglutaminase Assay Kit

Transglutaminases catalyze acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. The transglutaminase-catayzed covalent coupling of monodansylcadaverine into N,N-dimethylcasein produces a shift in intensity and wavelength of fluorescence of the dansyl group. The transglutaminase activity can be monitored online by measurement of the fluorescence (excitation wavelength 332 nm; emission wavelength 500 nm). The relative transglutaminase activity is shown by increase of fluorescence intensity over time.