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Fluorescein-12-UTP

ENZ-42834

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Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.

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Product Details

Alternative Name	Fluorescein-12-uridine-5’-triphosphate
Appearance	Yellow-green liquid and orange frozen solution.
Concentration	10mM
Emission Maximum	520 nm
Excitation Maximum	496 nm
Extinction Coefficient	85,000 M-1 cm-1 (496 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Formula	C₃₉H₄₁N₄O₂₂P₃
Formulation	Liquid. Solution in water.
MW	1010.7 (free acid)
Purity	≥93% (HPLC)
Purity Detail	Purified by ion-exchange chromatography.
Technical Info / Product Notes	Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Stable for at least one year after receipt when stored as recommended.
Handling	Protect from light. Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

The levels of the long noncoding RNA MALAT1 affect cell viability and modulate TDP-43 binding to mRNA in the nucleus: Balaji, A., Button, A. C., et al.; J. Biol. Chem. 301, 108207 (2025), Abstract
MALAT1 regulates mRNA processing through sequence dependent RNA-RNA and RNA-protein interactions: Balaji, A., Hall, S., et al.; Nucleic Acids Res. 53, gkaf784 (2025), Abstract
Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA: A. C. Button, et al.; RNA 30, 240 (2024), Reactant(s) E. coli, Abstract

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Fluorescein-12-UTP

ENZ-42834

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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