FLUOR DE LYS^®-Succinyl Green, Desuccinylase Substrate

The FLUOR DE LYS^®–Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5.  The assay procedure has two steps.  First, the FLUOR DE LYS^®–Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.).  Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS^® Developer (Prod. No. BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm).  The FLUOR DE LYS^®–Succinyl Green, Desuccinylase Substrate has longer wavelength excitation and emission than the AMC-based FLUOR DE LYS^®–Succinyl, Desuccinylase Substrate (Prod. No. BML-KI590). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.