FLUOR DE LYS^®-Succinyl, Desuccinylase Substrate

The FLUOR DE LYS^®–Succinyl, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5.  The assay procedure has two steps.  First, the FLUOR DE LYS^®– Succinyl, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.).  Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS^® Developer (Prod. No. BML-KI105) produces a free AMC fluorophore which can be monitored with standard fluorometers (Ex. 350-380nm / Em. 450-480nm).