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FLUOR DE LYS® SIRT5 fluorometric drug discovery assay kit

BML-AK513

6 citations

  • BML-AK513-0001   —   100 tests
    $838.00
  • Easy-to-use kits (two-step) for screening SIRT5 inhibitors/activators; includes enough active enzyme for entire plate
  • Includes optimal substrate selected from a panel of succinylated sites
  • 96-well plate included, but can be adapted to higher well format
  • Control inhibitors included
  • Suitable for high-throughput screening (Z’-factors >0.73)
  • Optimal, specific SIRT5 substrate means low enzyme concentration, making ‘hit’ validation easy

The SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS® system have also been successfully employed in other formats, including cuvettes and 384-well plates. The SIRT5 Fluorometric Activity Assay is based on the unique FLUOR DE LYS®-Succinyl Substrate/Developer combination. The assay procedure has two steps. The FLUOR DE LYS®-Succinyl Substrate, which comprises a single lysine residue, -succinylated on its side-chain, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS®-Succinyl sensitizes it so that, in the second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Use of a succinylated, rather than acetylated substrate with SIRT5 results in readily observed saturation kinetics and a greater than 1000-fold increase in assay sensitivity.

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Regulatory Status

RUO – Research Use Only