FLUOR DE LYS^®-Green substrate

The FLUOR DE LYS^®-Green substrate offers a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS^®-Green Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS^® Developer (Prod. No. BML-KI105) produces a fluorophore. The FLUOR DE LYS^®-Green substrate has a higher wavelength excitation and emission than the original FLUOR DE LYS^® substrate (Prod. No. BML-KI104). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.