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FLUOR DE LYS®-Green HDAC fluorometric activity assay kit

BML-AK530

6 citations

ELISA kit
  • BML-AK530-0001   —   96 wells
    $520.00

An improved FLUOR DE LYS® HDAC assay with FLUOR DE LYS®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The FLUOR DE LYS®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes. It comes in a convenient 96-well format, with all reagents necessary for fluorescent HDAC or sirtuin activity measurements and calibration of the assay. The FLUOR DE LYS®-Green HDAC assay is based on the FLUOR DE LYS®-Green substrate and FLUOR DE LYS® developer combination. The FLUOR DE LYS® system (Fluorogenic Histone deAcetylase Lysyl Substrate/Developer) is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS®-Green substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® developer produces a fluorophore.

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Regulatory Status

RUO – Research Use Only