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FLUOFORTE® Reagent

ENZ-52014

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Calcium dye

Used in a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Fluorescent calcium indicator dye developed to improve cell loading and calcium response while maintaining the convenient Fluo-3, Fluo-4 and Calcium 4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Relative to the cited dyes, FLUOFORTE^® AM dye yields the brightest signal and largest assay window.

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Product Details

Appearance	Lyophilized, orange.
Application	Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
Application Notes	Provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets
Purity	≥95% (HPLC)
Solubility	Soluble in DMSO.
Technical Info / Product Notes	The FLUOFORTE^® Reagent is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Handling & Storage

Handling	Protect from light. Avoid freeze/thaw cycles.
Short Term Storage	-20°C
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

G(1-5)-EM2, a multi-targeted agonist to opioid and growth hormone secretagogue receptors exhibited nontolerance forming antinociceptive effects in a mouse model of burn pain: S. Cheng, et al.; Eur. J. Pharmacol. 986, 177148 (2025), Abstract
Novel chimeric peptides based on endomorphins and ghrelin receptor antagonist produced supraspinal antinociceptive effects with reduced acute tolerance in mice: B. Wu, et al.; Biochimie 228, 58 (2025), Abstract
Evidence that the cold- and menthol-sensing functions of the human TRPM8 channel evolved separately: D.D. Luu, et al.; Sci. Adv. 10, eadm9228 (2024), Abstract
pH-sensing GPR68 inhibits vascular smooth muscle cell proliferation through Rap1A: M.D. Williams, et al.; Am. J. Physiol. Heart Circ. Physiol. 327, H1210 (2024), Abstract
ScreenDMT reveals DiHOMEs are replicably inversely associated with BMI and stimulate adipocyte calcium influx: J.M. Dreyfuss, et al.; Commun. Biol. 7, 996 (2024), Abstract
The endoplasmic reticulum pool of Bcl-xL prevents cell death through IP3R-dependent calcium release: R. Gadet, et al.; Cell Death Discov. 10, 346 (2024), Abstract
Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth: N.V. Simwela, et al.; Nat. Commun. 15, 9322 (2024), Abstract
Engineering Supramolecular Nanofiber Depots from a Glucagon-Like Peptide‑1 Therapeutic: W. Chen et al.; ACS Nano 18, 31274 (2024), Abstract
Pre-conditioning of gingival epithelial cells with sub-apoptotic concentrations of curcumin prevents pro-inflammatory cytokine release: M.M. Grant, et al.; J. Periodontal Res. 58, 634 (2023), Abstract
TRPV2, a novel player in the human ovary and human granulosa cells: K. Eubler, et al.; Mol. Hum. Reprod. 29, gaad029 (2023), Abstract