Fluo-3 (ultra pure)

Fluo-3 is widely used in flow cytometry, confocal laserscanning microscopy and fluorescence spectroscopy. The probe has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Once inside the cell, Fluo-3 becomes fluorescent, in the presence of free Ca (II). Fluo-3 is a long wavelength calcium probe (488 nm excitable). The longer wavelength of absorption is convenient for minimizing photodamage in cells. Wavelength Maxima: Excitation 506nm, Emission 526nm