FLUO 3/AM

ALX-620-003

Datasheet
Manual
SDS
CofA

Already have an account?

Fluorescent probe for Ca²⁺. Cell permeable.

Cell permeable analog of FLUO 3. Fluorescent probe for Ca²⁺. After crossing the membrane, the product is quickly metabolized by cytoplasmic esterases to the membrane impermeant FLUO 3.

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

Product Details

Alternative Name	1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2′-amino-5′-methylphenoxy)ethane-N,N,N’,N’-tetraacetic acid, pentaacetoxy-methyl ester
Appearance	Red solid.
CAS	121714-22-5
Formula	C₅₁H₅₀Cl₂N₂O₂₃
Identity	Determined by 1H-NMR and fluorescence.
MW	1129.9
Purity	≥85% (HPLC)
Solubility	Soluble in DMSO (0.9mg/ml) and acetonitrile (2mg/ml).

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Neuronal extracellular vesicles influence the expression, degradation and oligomeric state of fructose 1,6-bisphosphatase 2 in astrocytes affecting their glycolytic capacity: Hajka, D., Budziak, B., et al.; Sci. Rep. 14, 20932 (2024), Abstract
The Us3 Protein of Herpes Simplex Virus 1 Inhibits T Cell Signaling by Confining LAT Activation via TRAF6: Y. Yang, et al.; J. Biol. Chem. 290, 15670 (2015), Application(s): Cell Culture, Abstract — Full Text
Recombinant human tumor necrosis factor alpha induces calcium oscillation and calcium-activated chloride current in human neutrophils. The role of calcium/calmodulin-dependent protein kinase: M.A. Schumann, et al.; J. Biol. Chem. 268, 2134 (1993), Abstract — Full Text
Control of mitochondrial matrix calcium: studies using fluo-3 as a fluorescent calcium indicator: A. Saavedra-Molina, et al.; BBRC 167, 148 (1990), Abstract
Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator: P.A. Vadenberghe & J.L. Ceuppens; J. Immunol. Meth. 127, 197 (1990), Abstract
Glutamate induces calcium waves in cultured astrocytes: long-range glial signaling: A.H. Cornell-Bell, et al.; Science 247, 470 (1990), Abstract
Photochemically generated cytosolic calcium pulses and their detection by fluo-3: J.P.Y. Kao, et al.; J. Biol. Chem. 264, 8179 (1989), Abstract — Full Text

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Specific Protocol

General Protocol (for Human T cells)

Reagents:
2mM Fluo 3/AM/DMSO (1mg Fluo 3/AM in 442μl DMSO)
Pluronic F127
Hanks-balanced salt solution (HBSS)
HEPES buffer saline (10mM HEPES, 1mM Na₂HPO₄, 137mM NaCl, 5mM KCl, 1mM CaCl₂, 0.5mM MgCl₂, 5mM glucose, 0.1% BSA, pH 7.4)

Protocol:

Add 16.5mg Pluronic F127 to Fluo 3/AM/DMSO solution. Pluronic F127 prevents aggregation of Fluo 3/AM in HBSS and helps uptake with cells.
Dilute the Fluo 3/AM solution with HBSS to prepare 4μM Fluo 3/AM working solution.
Add the Fluo 3/AM working solution to the cells, and incubate at 37°C for 20min.
Add 5 volumes of HBSS containing 1% fetal calf serum, and continue the incubation for another 40min.
Wash the cells 3 times with HEPES buffer saline. Then resuspend the cells to prepare 1x10⁵ cells/ml solution using HEPES buffer saline.
Incubate at 37°C for 10min. Then use the cells for fluorescent calcium ion detection.
Monitor the fluorescence at 528nm (excitation: 490-500nm)

Cell staining conditions differ by cell types, so it is necessary to optimize the conditions for each experiment.

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

English (EU) SDS

If your language is not available please fill out the SDS request form

Your Cart