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FMS-related tyrosine kinase 3 ligand (Flt-3 ligand) is a growth factor important for the proliferation of hematopoietic cells. Flt-3 ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like tyrosine kinase-3 (Flt-3). Flt-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt-3 ligand can stimulate the proliferation of cells expressing mouse Flt-3 receptors.
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Figure 2: Serial dilutions of Human FLT-3 Ligand, starting at 100 ng/mL, were added to OCI-AML5 cells. After 60 hours, proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human Flt-3 ligand has a predicted MW of 17.6 kDa.
| Regulatory Status |
RUO – Research Use Only |
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Related Products
| Alternative Name | Interleukin-3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor |
|---|---|
| Purity | ≥95% (Reducing and Non-reducing SDS-PAGE) |
| Source | Produced in E. coli. Non-glycosylated protein, containing 134 amino acids. |