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FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with the adapter protein, FADD, and caspase-8 and –10, and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R and TNFR1.
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This antibody is covered by our Worry-Free Guarantee.
Western blot analysis of FLIP (human), mAb (7F10) (Prod. No. ALX-804-961): Lane 1: MW marker, Lane 2&4: HeLa cell lysate, Lane 3&5: Jurkat cell lysate. The two membranes were blotted with 7F10 or NF6 clones at 2µg/ml separately. The result showed they detected very similar pattern in these cell lysates.
Product Details
| Alternative Name |
CASPER, CLARP, I-FLICE, Usurpin, CFLAR |
|---|---|
| Application |
ELISA, Flow Cytometry, IHC, WB |
| Clone |
7F10 |
| Formulation |
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
| Host |
Mouse |
| Immunogen |
Recombinant human FLIP. |
| Isotype |
IgG1 |
| Purity Detail |
Protein G-affinity purified. |
| Recommendation Dilutions/Conditions |
Western Blot (1:500)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Source |
Purified from ascites. |
| Species Reactivity |
Human |
| Technical Info / Product Notes |
Cited samples: |
| UniProt ID |
O15519 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Handling |
Avoid freeze/thaw cycles. |
|---|---|
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Activation of the integrated stress response and loss of cFLIPL under glutamine limitation induce IL-8 gene expression and secretion in glutamine-dependent tumor cells: Mora-Molina, R., Fernández-Farrán, F. J., et al.; Cell Death Discov. 11, 332 (2025), Abstract
- IRE1 RNase controls CD95-mediated cell death: Pelizzari-Raymundo, D., Maltret, V., et al.; EMBO Rep. 25, 1792 (2024), Abstract
- Glutamine-mediated epigenetic regulation of cFLIP underlies resistance to TRAIL in pancreatic cancer: Kim, J. H., Lee, J., et al.; Exp. Mol. Med. 56, 1013 (2024), Abstract
- Role of the YAP/TAZ-TEAD Transcriptional Complex in the Metabolic Control of TRAIL Sensitivity by the Mevalonate Pathway in Cancer Cells.: El Yousfi, Y., Mora-Molina, R., et al.; Cells 12, (2023), Application(s): WB / Reactant(s): Human, Abstract
- DR5 Up-Regulation Induced by Dichloroacetate Sensitizes Tumor Cells to Lipid Nanoparticles Decorated with TRAIL: J.M. Brualla, et al.; J. Clin. Med. 12, 608 (2023), Abstract
- Lucanthone, Autophagy Inhibitor, Enhances the Apoptotic Effects of TRAIL through miR-216a-5p-Mediated DR5 Upregulation and DUB3-Mediated Mcl-1 Downregulation: J.Y. Yoon, et al.; Int. J. Mol. Sci. 23, 17 (2022), Abstract
- PKR and TLR3 trigger distinct signals that coordinate the induction of antiviral apoptosis: W. Zuo, et al.; Cell Death Dis. 13, 707 (2022), Abstract
- Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5: S.M. Woo, et al.; Cell Death Dis. 13, 821 (2022), Abstract
- Rafoxanide sensitizes colorectal cancer cells to TRAIL-mediated apoptosis: Laudisi, F., Pacifico, T., et al.; Biomed. Pharmacother. 155, 113794 (2022), Abstract
- Limiting glutamine utilization activates a GCN2/TRAIL-R2/Caspase-8 apoptotic pathway in glutamine-addicted tumor cells.: Yerbes, R., Mora-Molina, R., et al.; Cell Death Dis. 13, 906 (2022), Application(s): WB / Reactant(s): Human, Abstract
- Dapagliflozin induces apoptosis by downregulating cFILPL and increasing cFILPS instability in Caki-1 cells.: Jang, J. H., Lee, T. J., et al.; Oncol. Lett. 24, 401 (2022), Application(s): WB, Abstract
- The deubiquitinase Usp27x as a novel regulator of cFLIPL protein expression and sensitizer to death-receptor-induced apoptosis.: Häcker, G., Weber, A., et al.; Apoptosis 27, 112 (2022), Application(s): WB / Reactant(s): Human, Abstract
- Exploring the MEN1 dependent modulation of caspase 8 and caspase 3 in human pancreatic and murine embryo fibroblast cells: N. Wagener, et al.; Apoptosis , (2021), Abstract
- Pioglitazone mediates apoptosis in Caki cells via downregulating c-FLIP(L) expression and reducing Bcl-2 protein stability.: Kim, J. Y., Jang, J. H., et al.; Oncol. Lett. 22, 743 (2021), Application(s): WB, Abstract
- Selectively targeting myeloid-derived suppressor cells through TRAIL receptor 2 to enhance the efficacy of CAR T cell therapy for treatment of breast cancer: S.A. Nalawade, et al.; J. Immunother. Cancer 9, 3237 (2021), Abstract
- Potent pro-apoptotic combination therapy is highly effective in a broad range of cancers: A. Montinaro, et al.; Cell Death Differ. 10, 1038 (2021), Abstract
- 3-O-acetylrubianol C (3AR-C) induces RIPK1-dependent programmed cell death by selective inhibition of IKKβ: K. Kang, et al.; FASEB J. 34, 4369 (2020), Abstract
- The Histone Lysine-specific Demethylase 1 Inhibitor, SP2509 Exerts Cytotoxic Effects against Renal Cancer Cells through Downregulation of Bcl-2 and Mcl-1: Wu, K., Woo, S. M., et al.; J. Cancer Prev. 25, 79 (2020), Abstract
- Co-treatment of birinapant with TRAIL synergistically induces apoptosis by downregulating cFLIP(L) in MDA-MB-453 cell lines: E.J. Park, et al.; Biochem. Biophys. Res. Commun. 533, 289 (2020), Abstract
- Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells: A. Wentao, et al.; J. Exp. Clin. Cancer Res. 38, 181 (2019), Abstract — Full Text
- The Third-Generation EGFR Inhibitor, Osimertinib, Promotes c-FLIP Degradation, Enhancing Apoptosis Including TRAIL-Induced Apoptosis in NSCLC Cells with Activating EGFR Mutations: Shi, P., Zhang, S., et al.; Transl. Oncol. 12, 705 (2019), Abstract
- Involvement of up-regulation of death receptors and Bim in Hispolon-mediated TNF-related apoptosis-inducing ligand sensitization in human renal carcinoma: J.M. Yun, et al.; J. Cancer Prev. 24, 155 (2019), Application(s): Western-blot using Caki cells, Abstract — Full Text
- The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells: M. Li, et al.; J. Exp. Clin. Cancer Res. 38, 64 (2019), Reactant(s) Human, Abstract — Full Text
- The transcription factor SP3 drives TNF-α expression in response to Smac mimetics: S.T. Beug, et al.; Sci. Signal. 12, eaat9563 (2019), Abstract
- TRAIL-Induced Apoptosis in TRAIL-Resistant Breast Carcinoma Through Quercetin Cotreatment: J.M. Manouchehri, et al.; Breast Cancer (Auckl.) 12, 1178223417749850 (2018), Application(s): Western blot, Abstract — Full Text
- Acquired Resistance of ER-Positive Breast Cancer to Endocrine Treatment Confers an Adaptive Sensitivity to TRAIL through Posttranslational Downregulation of c-FLIP: L. Piggott, et al.; Clin. Cancer Res. 24, 2452 (2018), Abstract
- Differential expression patterns of the short and long isoform of cFLIP on FasL‑mediated apoptosis: H.J. Um, et al.; Oncol. Rep. 39, 2443 (2018), Abstract
- Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells: K.K. Kolluri, et al.; Elife 7, e30224 (2018), Application(s): WB, Abstract — Full Text
- Metformin-induced apoptosis facilitates degradation of the cellular caspase 8 (FLICE)-like inhibitory protein through a caspase-dependent pathway in human renal cell carcinoma A498 cells: J.H. Jang, et al.; Oncol. Lett. 16, 2030 (2018), Abstract — Full Text
- Sensitization of renal carcinoma cells to TRAIL-induced apoptosis by rocaglamide and analogs: Nalli, A. D., Brown, L. E., et al.; Sci. Rep. 8, 17519 (2018), Abstract
- Glutamine metabolism regulates FLIP expression and sensitivity to TRAIL in triple-negative breast cancer cells: Mauro-Lizcano, M., López-Rivas, A., et al.; Cell Death Dis. 9, 205 (2018), Abstract
- Differential expression patterns of the short and long isoform of cFLIP on FasL-mediated apoptosis: H.J. Um, et al.; Oncol. Rep. 39, 2443 (2018), Abstract
- cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition: C. Padmanabhan, et al.; Oncotarget 8, 101072 (2017), Application(s): WB / Reactant(s) Human, Abstract — Full Text
- Secretory stressors induce intracellular death receptor accumulation to control apoptosis: van Raam, B. J., Lacina, T., et al.; Cell Death Dis. 8, e3069 (2017), Abstract
- Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways: M.C. Tanzer, et al.; Cell Death Differ. 24, 481 (2017), Application(s): WB / Reactant(s) Human, Abstract — Full Text
- Role of p63 and p73 isoforms on the cell death in patients with hepatocellular carcinoma submitted to orthotopic liver transplantation: R. Gonzalez, et al.; PLoS One 12, e0174326 (2017), Abstract — Full Text
- Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome” Complex upon TRAIL Stimulation”””: C.M. Henry, et al.; Mol. Cell 65, 715 (2017), Abstract
- Autophagy-Mediated Degradation of IAPs and c-FLIP(L) Potentiates Apoptosis Induced by Combination of TRAIL and Chal-24: J. Xu, et al.; J. Cell. Biochem. 117, 1136 (2016), Abstract — Full Text
- Quercetin sensitizes pancreatic cancer cells to TRAIL-induced apoptosis through JNK-mediated cFLIP turnover: J.H. Kim, et al.; Int. J. Biochem. Cell Biol. 78, 327 (2016), Application(s): Western blot; cell lysates, Abstract
- Overcoming resistance to TRAIL-induced apoptosis in solid tumor cells by simultaneously targeting death receptors, c-FLIP and IAPs: Y. Huang, et al.; Int. J. Oncol. 49, 153 (2016), Application(s): Western-blot using U2OS cells, Abstract — Full Text
- Targeting of BCL2 family proteins with ABT-199 and homoharringtonine reveals BCL2- and MCL1-dependent subgroups of diffuse large B-cell lymphoma: M. Klanova, et al.; Clin. Cancer Res. 22, 1138 (2016), Application(s): Western-blot using BJAB and OCI-Ly7 cells, Abstract
- Inhibition of BET bromodomain-dependent XIAP and FLIP expression sensitizes KRAS-mutated NSCLC to pro-apoptotic agents: Klingbeil, O., Lesche, R., et al.; Cell Death Dis. 7, e2365 (2016), Abstract
- cFLIPL Interrupts IRF3-CBP-DNA Interactions To Inhibit IRF3-Driven Transcription: Gates, L. T., Shisler, J. L., et al.; J. Immunol. 197, 923 (2016), Abstract
- A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors: C.J. Heinrich; PLoS One 11, e0165192 (2016), Abstract
- Galangin sensitizes TRAIL-induced apoptosis through down-regulation of anti-apoptotic proteins in renal carcinoma Caki cells: M.A. Han, et al.; Sci. Rep. 6, 18642 (2016), Reactant(s) Human, Mouse, Abstract — Full Text
- Infection of epithelial cells with Chlamydia trachomatis inhibits TNF-induced apoptosis at the level of receptor internalization while leaving non-apoptotic TNF-signalling intact: C. Waguia Kontchou, et al.; Cell. Microbiol. 18, 1583 (2016), Abstract
- YM155 sensitizes TRAIL-induced apoptosis through cathepsin S-dependent down-regulation of Mcl-1 and NF-κB-mediated down-regulation of c-FLIP expression in human renal carcinoma Caki cells: S.M. Woo, et al.; Oncotarget 7, 61520 (2016), Reactant(s) Human, Abstract — Full Text
- 6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression: M.A. Han, et al.; Chem. Biol. Interact. 228, 69 (2015), Abstract
- G2/M Arrest Sensitises Erythroid Leukemia Cells to TRAIL-induced Apoptosis: S. Cruet-Hennequart, et al.; J. Leuk. 3, 174 (2015), Application(s): Western Blot, Full Text
- Hyperthermia restores apoptosis induced by death receptors through aggregation-induced c-FLIP cytosolic depletion: Morlé, A., Garrido, C., et al.; Cell Death Dis. 6, e1633 (2015), Abstract
- Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis: S. Shi, et al.; Oncotarget 6, 1640 (2015), Abstract — Full Text
- FAS-ligand regulates differential activation-induced cell death of human T-helper 1 and 17 cells in healthy donors and multiple sclerosis patients: M.T. Cencioni, et al.; Cell. Death Dis. 6, e1741 (2015), Application(s): Western Blot, Abstract — Full Text
- Helicobacter pylori sensitizes TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human gastric epithelial cells through regulation of FLIP: W.C. Lin, et al.; Cell Death Dis. 5, e1109 (2014), Application(s): Western blot using human gastric adenocarcinoma cell line AGS, Abstract — Full Text
Related Products
| Alternative Name | CASPER, CLARP, I-FLICE, Usurpin |
|---|---|
| Application | ICC, WB |
| Host | Rabbit |
| Species Reactivity | Human, Mouse, Rat |
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