FGFR/β-Klotho Reporter Assay System

INDIGO’s Human Fibroblast Growth Factor Receptor Receptor 1c and β-Klotho (FGFR/β-Klotho) assay utilizes proprietary human cells that have been engineered to provide constitutive expression of the Human Fibroblast Growth Factor Receptor 1c and β-Klotho. FGFR1c and β-Klotho are both single-pass transmembrane proteins. FGFR1c has an extracellular ligand-binding domain, transmembrane domain, and intracellular tyrosine kinase domain. It has been established that FGFR association with the co-receptor β-Klotho generates a scaffold that is essential for endocrine growth factor binding interactions, such as those with FGF-21 and FGF-19. Following growth factor binding, the activated tyrosine kinase activities of the FGFR initiate intracellular signaling cascades that may include RAS-MAPK, PI3-AKT, PLCγ and/or STAT pathways. It is FGFR1c/β-Klotho signal transduction via the RAS-MAPK-ERK1/2 cascade that is exploited by the reporter cells in this assay.