Fc (human):TNF-α (soluble) (human), (recombinant)

Tumor necrosis factor α, TNFSF 2

0.1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.).

Lyophilized. Contains PBS.

~48kDa (calculated), ~52kDa (observed)

≥90% (SDS-PAGE)

Purified by multi-step chromatography.

Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).

Historical data has shown that TNFα induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer).

P01375