Fc (human):CD40L (soluble) (mouse), (recombinant)

CD154, TNFSF 5, gp39

ELISA: binds to human CD40:COMP at 10-100 ng/ml.

0.1mg/ml after reconstitution.

<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).

Lyophilized. Contains PBS.

~58kDa (SDS-PAGE).

≥90% (SDS-PAGE)

Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).

Binds to human and mouse CD40.

Historical data has shown that Fc (human):CD40L (mouse) stimulates the proliferation of mouse B cells and dendritic cells. Note: Does not require an enhancer.

P27548