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Regulatory Status |
RUO – Research Use Only |
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Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
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Figure: Proliferation of human dendritic cells (DC) induced by Fc (human):CD40L, Soluble (human) (rec.) (Prod. No. ALX-522-076).Method: Human DC were derived from GMCSF/IL-4 treated monocytes. The so obtained DC, 105 cells/assay, were incubated with 3µg/ml Fc (human):CD40L, Soluble (human) (rec.) (A), 3µg/ml boiled Fc (human):CD40L, Soluble (human) (rec.) (B), 3µg/ml control:Fc (C) and media (D). After 18 hours, DC maturation was analyzed by immunostaining with a CD83-specific MAb and cytofluorimetry analysis. Indicated are the percentages of activated DC.”””,Fc (human):CD40L (soluble) (human)
Alternative Name |
CD154, TNFSF 5, gp39 |
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Application Notes |
ELISA: binds to human CD40:COMP at 2-50 ng/ml. |
Concentration |
0.1mg/ml after reconstitution. |
Endotoxin Content |
<0.1EU/µg purified protein (LAL test; BioWhittaker). |
Formulation |
Lyophilized. Contains PBS. |
MW |
~58kDa (SDS-PAGE). |
Purity |
≥90% (SDS-PAGE) |
Reconstitution |
Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
Source |
Produced in HEK 293 cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa). |
Specificity |
Binds to human CD40. |
Technical Info / Product Notes |
Historical data has shown that Fc (human):CD40L stimulates the proliferation of human dendritic cells. Note: Does not require an enhancer. |
UniProt ID |
P29965 |
Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. |
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Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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