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FasL, soluble (human) (recombinant) set

ALX-850-014

25 citations

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Product Details

Alternative Name	APO-1L, CD95L, CD178, TNFSF 6
Application	FUNC
Application Notes	Kills Fas-sensitive cells at concentrations of >1ng/ml in the presence of cross-linking enhancer.
Concentration	FasL: 0.1mg/ml after reconstitutionEnhancer: 1mg/ml
Contents	10µg of FasL, Soluble (human) (recombinant) (Prod. No. ALX-522-001) and 4×50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity (induction of apoptosis) by ~50-fold to a concentration range of 1-5ng/ml.
Formulation	FasL: Lyophilized. Contains PBS. Enhancer: Liquid. In PBS, pH7.4, containing 50% glycerol. Contains no preservatives.
Purity	≥95% (SDS-PAGE)
Reconstitution	Reconstitute FasL with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
Source	Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of FasL, Soluble (human) (recombinant) is similar to natural human FasL.
Specific Activity	ED₅₀: 1ng/ml (A20 cells)
Specificity	FasL binds to human, mouse and rat Fas (CD95).
Technical Info / Product Notes	FLAG is a registered trademark of Sigma-Aldrich Co.
UniProt ID	P48023

Handling & Storage

Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Advanced multiparametric image spectroscopy and super-resolution microscopy reveal a minimal model of CD95 signal initiation: Bartels, N., van der Voort, N. T. M., et al.; Sci. Adv. 10, eadn3238 (2024), Abstract
A Minimal Model of CD95 Signal Initiation Revealed by Advanced Super-resolution and Multiparametric Fluorescence Microscopy: Bartels, N., van der Voort, N. T. M., et al.; bioRxiv , (2022)
The non-apoptotic function of Caspase-8 in negatively regulating the CDK9-mediated Ser2 phosphorylation of RNA polymerase II in cervical cancer: R. Mandal, et al.; Cell. Mol. Life Sci. 79, 597 (2022), Abstract
Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis: Tummers, B., Mari, L., et al.; Immunity 52, 994 (2020), Abstract
Amplification of CD95 activation by caspase 8-induced endosomal acidification in rat hepatocytes: R. Reinehr, et al.; J. Biol. Chem. 283, 2211 (2008), Abstract — Full Text
Fas (CD95) induces macrophage proinflammatory chemokine production via a MyD88-dependent, caspase-independent pathway: W.A. Altemeier, et al.; J. Leukoc. Biol. 82, 721 (2007), Abstract — Full Text
p21CIP1/WAF1 controls proliferation of activated/memory T cells and affects homeostasis and memory T cell responses: C.F. Arias, et al.; J. Immunol. 178, 2296 (2007), Abstract
Retinoic acid inhibits the proliferative response induced by CD40 activation and interleukin-4 in mantle cell lymphoma: M. Guidoboni, et al.; Cancer Res. 65, 587 (2005), Abstract — Full Text
CD1d-unrestricted human NKT cells release chemokines upon Fas engagement: M. Giroux and F. Denis; Blood 105, 703 (2005), Abstract — Full Text
The role of p53 and Fas in a model of acute murine graft-versus-host disease: S. Yada, et al.; J. Immunol. 174, 1291 (2005), Abstract — Full Text

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FasL (soluble) (human), (recombinant)

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Fas ligand

44 citations

Alternative Name	APO-1L, CD95L, CD178, TNFSF 6
Purity	≥95% (SDS-PAGE)
Source	Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of rhsFasL is similar to that of natural human FasL.

Enhancer for Ligands

ALX-804-034

Enhances biological activity of TRAIL, soluble (human) (recombinant) and FasL, soluble (human) (recombinant).

19 citations

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FasL, soluble (human) (recombinant) set

ALX-850-014

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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