Home Shop FasL (mouse) monoclonal antibody (A11)

FasL (mouse) monoclonal antibody (A11)

ALX-804-009

11 citations

Datasheet
Manual
SDS
CofA

Already have an account?

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

This antibody is covered by our Worry-Free Guarantee.

FasL (mouse) monoclonal antibody (A11) Schematic structure of immunogen — Schematic structure of mouse FasL (peptide, aa. 196-220)

FasL (mouse) monoclonal antibody (H11) (biotin conjugate) Schematic structure of immunogen — **Figure:** Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody.
**Method:** Cryostat sections (6-8µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 µg/ml of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
**Paraffin sections:** Dewax in xylene (2×10 min), EtOH 100% (2×10 min), block endogeneous peroxidase (0.6% H₂O₂ in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H₂0, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2×5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/ml antibody, wash in PBS, incubate in anti-rat IgM HRP (ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC).”””,FasL (mouse) monoclonal antibody (A11) Immunohistochemistry,FasL (mouse) monoclonal antibody (A11) Immunohistochemistry,yes
ALX-804-010,804-010.gif,http://static.enzolifesciences.com/fileadmin/files/image/804-010.gif,Schematic structure of mouse FasL (peptide

Product Details

Alternative Name	CD178, CD95L, APO-1L, TNFSF 6
Application	Flow Cytometry, IHC (FS), IHC (PS)
Application Notes	For Western blot, we recommend MAb to FasL (mouse) (H11) (Prod. No. ALX-804-010).
Clone	A11
Formulation	Liquid. Purified antibody in PBS containing 0.02% sodium azide.
Host	Rat
Immunogen	Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
Isotype	IgM
Source	Purified from hybridoma tissue culture supernatant.
Species Reactivity	Mouse
UniProt ID	P41047
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling	Avoid freeze/thaw cycles.
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Loss of functional Fas ligand enhances intestinal tumorigenesis in the Min mouse model: Fingleton, B., Carter, K. J., et al.; Cancer Res. 67, 4800 (2007), Abstract
Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions: I. Viard-Leveugle, et al.; J. Biol. Chem. 278, 16183 (2003), Abstract — Full Text
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia: M. Sata, et al.; J. Immunol. 166, 6964 (2001), Abstract
Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy: M. Sata, et al.; Arterioscler. Thromb. Vasc. Biol. 20, 309 (2000), Abstract
The regulation of CD95 ligand expression and function in CTL: J.H. Li, et al.; J. Immunol. 161, 3943 (1998), Abstract
Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis: Y. Refaeli, et al.; Immunity 8, 615 (1998), Abstract
Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?: P. Saas, et al.; J. Clin. Invest. 99, 1173 (1997), Abstract — Full Text
Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover: L.E. French, et al.; J. Cell. Biol. 133, 335 (1996), Abstract
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996), Abstract
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand: T. Renno, et al.; J. Exp. Med. 183, 431 (1996), Abstract

Related Products

FasL (mouse) monoclonal antibody (H11)

ALX-804-010

4 citations

Alternative Name	CD178, CD95L, APO-1L, TNFSF 6
Application	Flow Cytometry, ICC, WB
Host	Rat
Isotype	IgG2a
Species Reactivity	Mouse

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

English (EU) SDS

If your language is not available please fill out the SDS request form

Your Cart

Your Cart

FasL (mouse) monoclonal antibody (A11)

ALX-804-009

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

Change Country

Tell us about You

Tell us about your paper

Tell us about You

Tell us about your project