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Figure: Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells.
Method: (A) Dose response: Jurkat cells (1 x 105) were treated with agonistic anti-Fas 2R2 antibodies at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry.
(B) Time course of apoptosis: Jurkat cells (1 x 105) were treated with 1 µg/ml anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA.

Figure: Detection of Fas surface expression by FACS analysis.
Method: Normal L cells (open histogram) and cells stably overexpressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunstaining was obtained with an isotype matched IgG control.


Product Details
Alternative Name |
CD95, APO-1, TNFRSF 6 |
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Application |
Flow Cytometry, FUNC |
Application Notes |
Flow Cytometry |
Clone |
2R2 |
Formulation |
Liquid. In PBS containing 1% BSA. Contains no preservatives. |
Host |
Mouse |
Immunogen |
Mouse L cells stably overexpressing human Fas (CD95; APO-1). |
Isotype |
IgG3 |
Purity Detail |
Protein A-affinity purified. |
Recommendation Dilutions/Conditions |
Flow Cytometry, Functional Application (induces apoptosis – no cross-linking required). , – Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation., Recommended working dilutions are 1:200-1:1,000. |
Source |
Purified from concentrated hybridoma tissue culture supernatant. |
Species Reactivity |
Human |
UniProt ID |
P25445 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Handling |
Avoid freeze/thaw cycles. |
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Short Term Storage |
+4°C |
Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- IRE1 RNase controls CD95-mediated cell death: Pelizzari-Raymundo, D., Maltret, V., et al.; EMBO Rep. 25, 1792 (2024), Abstract
- IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000), Abstract
- Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999), Abstract — Full Text
- Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998), Abstract — Full Text
Last modified: May 29, 2024
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