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Immunofluorescence staining of FABP5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FABP5 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
FABP5 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate, 4 µL anti-FABP5 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-FABP5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Observed band size: 15 kDa.
Anti-FABP5 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Observed band size:15 kDa.
| Regulatory Status |
RUO – Research Use Only |
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