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Estrogen receptor α polyclonal antibody

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Alternative Name	ERα
Application	IHC (FS), WB
Application Notes	Detects a band of ~64kDa by Western blot.
Crossreactivity	Does not cross-react with ERβ.
Formulation	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
Host	Rabbit
Immunogen	Synthetic peptide corresponding to aa 1-21 (M1TMTLHTKASGMALLHQIQGN21) of N-terminal human ERα (estrogen receptor α). This sequence is completely conserved in rat and mouse ERα and is highly conserved in sheep, pig, dog, bovine and chicken ERα.
Purity Detail	Epitope-affinity purified IgG.
Recommendation Dilutions/Conditions	Immunohistochemistry (frozen sections, 1-5µg/ml)Western Blot (1µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Human, Rat
Specificity	Recognizes endogenous ERα.
UniProt ID	P03372
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Regulatory Status	RUO – Research Use Only

Histone deacetylase inhibitors down-regulate G-protein-coupled estrogen receptor and the GPER-antagonist G-15 inhibits proliferation in endometriotic cells.: Dedes, K. J., Fedier, A., et al.; Fertil. Steril. 100, 770 (2013), Application(s): WB / Reactant(s): Human, Abstract
Immunohistochemical localization of oestrogen receptor alpha in the various cell categories of chick embryo ovary: Civinini, A., Chimenti, C., et al.; Anat. Histol. Embryol. 39, 546 (2010), Abstract
Cytochromes 1A1/1B1- and catechol-O-methyltransferase-derived metabolites mediate estradiol-induced antimitogenesis in human cardiac fibroblast: R.K. Dubey, et al.; J. Clin. Endocrinol. Metab. 90, 247 (2005), Abstract — Full Text
Differential Regulation of Estrogen Receptor Subtypes alpha and beta in Human Aortic Smooth Muscle Cells by Oligonucleotides and Estradiol: F. Barchiesi, et al.; J. Clin. Endocrinol. Metab. 89, 2373 (2004), Abstract

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