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Estrogen receptor monoclonal antibody (h-151)

ADI-SRA-1000

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The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

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Estrogen receptor monoclonal antibody (h-151) Schematic structure — Human Estrogen Receptor domains.

Estrogen receptor monoclonal antibody (h-151) Western blot — Western blot analysis of ESR1: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa)

Product Details

Alternative Name	ESR
Application	ICC, IP, WB
Application Notes	Detects a band of ~67kDa by Western blot.
Clone	h-151
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
GenBank ID	M12674
Host	Mouse
Immunogen	Synthetic peptide corresponding to the sequence of human estrogen receptor, conjugated to KLH.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Immunoprecipitation (15µg/ml)Western Blot (1:250)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from mouse ascites.
Species Reactivity	Human, Rat
UniProt ID	P03372
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res. 31, 521 (2011), Application(s): Flow cytometry and immunofluorescence, Abstract
Serum-dependent effects of tamoxifen and cannabinoids upon C6 glioma cell viability: C.J. Fowler, et al.; Biochem. Pharmacol. 60, 1807 (2000), Application(s): WB using rat samples, Abstract
Rapid actions of estrogens in GH3/B6 pituitary tumor cells via a plasma membrane version of estrogen receptor-alpha: B. Gametchu, et al.; Steroids 64, 13 (1999), Application(s): ICC using human & rat samples, Abstract
An N-terminal inhibitory function, IF, suppresses transcription by the A-isoform but not the B-isoform of human progesterone receptors: K.B. Horwitz, et al.; J. Biol. Chem. 273, 5455 (1998), Application(s): WB using human samples, Abstract
AIB1, a steroid receptor coactivator amplified in breast cancer and ovarian cancer: P.S. Meltzer, et al.; Science 277, 965 (1997), Application(s): WB using human samples, Abstract

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Estrogen receptor monoclonal antibody (h-151)

ADI-SRA-1000

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Estrogen receptor monoclonal antibody (h-151)

ADI-SRA-1000

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