Home Shop Estrogen receptor α monoclonal antibody (C-542)

Estrogen receptor α monoclonal antibody (C-542)

ADI-SRA-1010

Datasheet
Manual
SDS
CofA

Already have an account?

The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

This antibody is covered by our Worry-Free Guarantee.

Estrogen receptor alpha monoclonal antibody (C-542) Schematic structure — Human Estrogen Alpha Receptor domains.

Estrogen receptor alpha monoclonal antibody (C-542) Immunohistochemistry — Immunohistochemistry analysis of human Breast tumor tissue stained with Estrogen receptor α monoclonal antibody (C-542), (Prod. No. ADI-SRA-1010), at 20µg/ml.

Estrogen receptor alpha monoclonal antibody (C-542) Western blot — Western blot analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).

Estrogen receptor alpha monoclonal antibody (C-542) Immunocytochemistry — Immunocytochemistry staining of HepG2 cells (Panel A) and MCF-7 (Panel B) with Estrogen receptor α monoclonal antibody (C-542) (Prod. No. ADI-SRA-1010) at 10µg/ml.

Product Details

Alternative Name	ESR
Application	ICC, IHC, IP, WB
Application Notes	Detects a band of 66.3kDa by Western blot.
Clone	C-542
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
GenBank ID	M12674
Host	Mouse
Immunogen	Synthetic peptide corresponding to the sequence near the C-terminus of human Estrogen Receptor α.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:500, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from ascites.
Species Reactivity	Hamster, Human, Mouse, Rat
UniProt ID	P03372
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Chronic exposure to benzyl butyl phthalate (BBP) alters social interaction and fear conditioning in male adult rats: alterations in amygdalar MeCP2, ERK1/2 and ERα: A. Betz, et al.; Neuro. Endocrinol. Lett. 34, 347 (2013), Abstract
Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res. 31, 521 (2011), Application(s): Flow cytometry and immunofluorescence, Abstract
Membrane estrogen receptor-α levels in MCF-7 breast cancer cells predict cAMP and proliferation responses: D. Zivadinovic, et al.; Breast Cancer Res. 7, R101 (2005), Application(s): Cell immunoseparation, Abstract
Regulation of the membrane estrogen receptor-alpha: role of cell density, serum, cell passage number, and estradiol: C.S. Watson, et al.; FASEB J. 16, 1917 (2002), Application(s): ICC using rat samples, Abstract
The novel isoform of the estrogen receptor-alpha cDNA (ERalpha isoform S cDNA) in the human testis: K. Hoshi, et al.; J. Steroid Biochem. Mol. Biol. 80, 299 (2002), Application(s): WB using human samples, Abstract
Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex: L.P. Freedman, et al.; J. Biol. Chem. 275, 20928 (2000), Application(s): IP, WB using human samples, Abstract
Estrogen receptor-dependent regulation of sensory neuron survival in developing dorsal root ganglion: D. Lindholm, et al.; PNAS 96, 10905 (1999), Application(s): ICC using rat samples, Abstract
Characterization of Brx, a novel Dbl family member that modulates estrogen receptor action: J. Segars, et al.; Oncogene 16, 2513 (1998), Application(s): IP, IHC using human samples, Abstract

Related Products

Goat anti-mouse IgG, polyclonal antibody (AP conjugate)

ADI-SAB-101

6 citations

Application	ELISA, WB
Host	Goat
Species Reactivity	Mouse

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Certificate of Analysis

Please enter the lot number as featured on the product label

Lot No. :

SDS

Enzo Life Science provides GHS Compliant SDS

English (EU) SDS

If your language is not available please fill out the SDS request form

Your Cart

Your Cart

Estrogen receptor α monoclonal antibody (C-542)

ADI-SRA-1010

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

Change Country

Tell us about You

Tell us about your paper

Tell us about You

Tell us about your project