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ERp57 polyclonal antibody

ADI-SPA-585

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ERp57 (also known as Grp58), a member of the Thioredoxin superfamily, is an ER protein with disulfide isomerase activity. It has been shown to increase after oncogenic transformation and to be a component of the MHC Class I peptide-loading complex.

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This antibody is covered by our Worry-Free Guarantee.

ERp57 polyclonal antibody Western blot — Western blot analysis of ERp57 (Grp58): Lane 1: MW marker, Lane 2: CHO-K1 lysate, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes.

ERp57 polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis of human colon tissue stained with ERp57, pAb at 10µg/ml.

Product Details

Alternative Name	PDIA3, ERp60, ERp61, GRP57, Grp58
Application	IHC (PS), WB
Application Notes	Detects a band of ~57kDa by Western blot.
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Gene/Protein Identifier	NM_017319 (RefSeq)
Host	Rabbit
Immunogen	Native rat liver ERp57.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat
UniProt ID	P11598
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Cnpy32xHA mice reveal neuronal expression of Cnpy3 in the brain: Md. M. Islam, et al.; J. Neurosci. Methods 383, 109730 (2023), Abstract
Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung: N. Chamberlain, et al.; Int. J. Mol. Sci. 23, 1078 (2022), Application(s): IP, Abstract
Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics.: Anathy, V., van der Vliet, A., et al.; Redox Biol. 22, 101129 (2019), Application(s): IP, Abstract
Protein disulfide isomerase plasma levels in healthy humans reveal proteomic signatures involved in contrasting endothelial phenotypes.: Martins-de-Souza, D., Debbas, V., et al.; Redox Biol. 22, 101142 (2019), Application(s): WB / Reactant(s): Human, Abstract
Comparative proteome analysis of monolayer and spheroid culture of canine osteosarcoma cells: C. Gebhard, et al.; J. Proteomics 177, 124 (2018), Application(s):IHC, Abstract
HLA-G peptide preferences change in transformed cells: impact on the binding motif: A.A. Celik, et al.; Immunogenetics 70, 485 (2018), Reactant(s) Human, Abstract — Full Text
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration: V. Castillo, et al.; PLoS One 10, e0136620 (2015), Application(s): Densitometry analysis Western blot, Abstract — Full Text
The day/night proteome in the murine heart.: Podobed, P., Pyle, W. G., et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 307, R121 (2014), Application(s): WB / Reactant(s): Mouse, Abstract
Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes: S. Badrinath, et al.; Haematologica 97, 98 (2012), Application(s): WB, WB post IP / Reactant(s) Human, Abstract — Full Text
The VP1 subunit of JC polyomavirus recapitulates early events in viral trafficking and is a novel tool to study polyomavirus entry.: Maginnis, M. S., Derdowski, A., et al.; Virology 428, 30 (2012), Application(s): WB / Reactant(s): Human, Abstract

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ERp57 polyclonal antibody

ADI-SPA-585

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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