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Erk1/2 polyclonal antibody

ADI-KAP-MA001

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The Extracellular regulated kinases, Erk-1 and Erk-2, both play a critical role in cellular proliferation. These extracellular kinases, are known to reside downstream of the Mitogen activated protein kinase/Extracellular protein kinases referred to as MEK. Both MEK-1 and MEK-2 phosphorylate and activate Erk on the conserved TEY motif within the activation loop of the kinase.

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This antibody is covered by our Worry-Free Guarantee.

Erk1/2 polyclonal antibody Flow Cytometry — Flow cytometry analysis of U251 cells using Erk1/2 pAb (right) and control rabbit IgG (left).

Erk1/2 polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis: Human placenta tissue stained with Erk1/2, pAb at 10µg/ml.

Erk1/2 polyclonal antibody Western blot — Western blot analysis of human A431 fibroblast cell lysate, probed with Erk1/2 pAb.

Product Details

Alternative Name	MAPK2/3, MAP kinase 2/3
Application	Flow Cytometry, IHC (PS), IP, WB
Application Notes	Detects a bands of ~42kDa (Erk2) and ~44kDa (Erk1) by Western blot.
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
GenBank ID	M61177
Host	Rabbit
Immunogen	Synthetic peptide corresponding to a portion of rat Erk1. The sequence is completely conserved in hamster.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (10µg/ml)Immunohistochemistry (paraffin sections, 1:50)Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Chicken, Drosophila, Human, Mouse, Rat, Sheep, Xenopus
UniProt ID	P21708
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The serine/threonine kinase Back seat driver prevents cell fusion to maintain cell identity: Yang, S., Johnson, A. N., et al.; Dev. Biol. 495, 35 (2023), Abstract
Photodynamic Therapy by Glucose Transporter 1-Selective Light Inactivation.: Miura, K., Wen, Y., et al.; ACS Omega 7, 34685 (2022), Application(s): WB / Reactant(s): Human, Abstract
Diverse and cancer type‑specific roles of the p53 R248Q gain‑of‑function mutation in cancer migration and invasiveness.: Olszewski, M. B., Pruszko, M., et al.; Int. J. Oncol. 54, 1168 (2019), Application(s): WB, Abstract
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Application(s): PAGE-MAP / Reactant(s) Human, Abstract
Constitutive Activation of STAT3 in Myeloma Cells Cultured in a Three-Dimensional, Reconstructed Bone Marrow Model.: Huang, Y. H., Molavi, O., et al.; Cancers (Basel) 10, (2018), Application(s): WB / Reactant(s): Human, Abstract
Constitutive Activation of STAT3 in Myeloma Cells Cultured in a Three-Dimensional, Reconstructed Bone Marrow Model: Wang, Q., Lai, R., et al.; Preprints.org , (2018), Reactant(s): Human
TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK: Ormonde, J. V. S., Li, Z., et al.; J. Immunol. 201, 3431 (2018), Abstract
EGF-mediated EGFR/ERK signaling pathway promotes germinative cell proliferation in Echinococcus multilocularis that contributes to larval growth and development: Z. Cheng, et al.; PLoS Negl. Trop. Dis. 11, e0005418 (2017), Abstract — Full Text
Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist: P. Yang, et al.; Exp. Neurol. 279, 178 (2016), Application(s): Immunoblotting, Abstract
Reduction of chronic allograft nephropathy by inhibition of extracellular signal-regulated kinase 1 and 2 signaling: C. Du, et al.; Am. J. Physiol. Renal Physiol. 295, F672 (2008), Application(s): WB, IHC using mouse tissue, Abstract