Home Shop ER-ID® Red assay kit (GFP-CERTIFIED®)

ER-ID^® Red assay kit (GFP-CERTIFIED^®)

ENZ-51026

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Widely cited Endoplasmic Reticulum staining with minimal toxicity to cells

Specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs)
Suitable for use with live or post-fixed cells
Highly resistant to photo-bleaching, concentration quenching and photoconversion
Suitable for use in conjunction with fluorescein- or coumarin-labeled antibodies

Enzo Life Sciences ER-ID^® Red assay kit (GFP-CERTIFIED^®) contains a red-emitting, cell-permeable small molecule organic probe to stain Endoplasmic Reticulum (ER) in live cell, or fixed cells. The dye emits in the Texas Red region of the visible light spectrum and can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes/fluorescent proteins in multi-color imaging and detection applications. The kit includes also the Hoechst 33342 dye for the nuclear staining.

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ER-ID® Red assay kit (GFP-CERTIFIED®) image — Figure 1: Live HeLa cells stained with ER-ID® Red dye (A), Hoechst dye (B) and resulting composite image (C).

Product Details

Alternative Name	Endoplasmic reticulum / Organelle
Application	Fluorescence microscopy
Application Notes	Enzo Life Sciences’ ER-ID^®Red assay kit (GFP-CERTIFIED^®) contains a novel endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.
Contents	ER-ID^® Red detection reagent: 50μl Hoechst 33342 nuclear stain: 50μl 10X assay buffer: 15ml
Quality Control	A sample from each lot of ER-ID^® Red assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivity of the ER-ID^® Red dye is evident.
Quantity	500 assays
Technical Info / Product Notes	The ER-ID^® Red assay kit (GFP-CERTIFIED^®) is a member of the CELLESTIAL^® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL^® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

Handling & Storage

Use/Stability	With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.
Handling	Protect from light. Avoid freeze/thaw cycles.
Short Term Storage	-20°C
Long Term Storage	-80°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

CDNF rescues human iPSCs-derived dopamine neurons through direct binding to unfolded protein response sensors PERK and IRE1α: Kovaleva, V., Shpironok, O., et al.; bioRxiv , (2024)
MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α: Kovaleva, V., Yu, L. Y., et al.; Cell Rep. 42, 112066 (2023), Abstract
DIAPH1-MFN2 interaction regulates mitochondria-SR/ER contact and modulates ischemic/hypoxic stress: G. Yepuri, et al.; Nat. Commun. 14, 6900 (2023), Abstract
CRISPR-Screen Identifies ZIP9 and Dysregulated Zn2+ Homeostasis as a Cause of Cancer-Associated Changes in Glycosylation: T.B. Rømer, et al.; Glycobiology , (2023), Abstract
Inhibition of TRPA1 Ameliorates Periodontitis by Reducing Periodontal Ligament Cell Oxidative Stress and Apoptosis via PERK/eIF2α/ATF-4/CHOP Signal Pathway: Liu, Q., Guo, S., et al.; Oxid. Med. Cell. Longev. 2022, 4107915 (2022), Abstract
Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells: A. Samy, et al.; Traffic 22, 425 (2021), Abstract
Norcantharidin combined with paclitaxel induces endoplasmic reticulum stress mediated apoptotic effect in prostate cancer cells by targeting SIRT7 expression: M.H. Wu, et al.; Environ. Toxicol. 36, 2206 (2021), Abstract
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1α: Kovaleva, V., Yu, L., et al.; bioRxiv , (2020)
Decoy receptor 3 promotes preosteoclast cell death via reactive oxygen species-induced fas ligand expression and the IL-1α/IL-1 receptor antagonist pathway: Y.J. Peng, et al.; Mediators. Inflamm. 2020, 1237281 (2020), Application(s): Fluorescence microscopy, Abstract — Full Text
TRPV1 antagonist DWP05195 induces ER stress-dependent apoptosis through the ROS-p38-CHOP pathway in human ovarian cancer cells: Y.Y. Wang, et al.; Cancers (Basel) 12, 1702 (2020), Application(s): Flow cytometry and fluorescence microscopy, Abstract — Full Text

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ER-ID^® Red assay kit (GFP-CERTIFIED^®)

ENZ-51026

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

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ER-ID® Red assay kit (GFP-CERTIFIED®)

ENZ-51026

Product Details

Handling & Storage

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LYSO-ID® Red detection kit (GFP-CERTIFIED®)

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Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

SDS

ER-ID^® Red assay kit (GFP-CERTIFIED^®)

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