EQ1 NHS ester

• Commonly used in molecular biology FRET applications to quench fluorophores
• NHS ester quencher dye can be conjugated to amine-labeled probes
• Enable multiplexing of probes with the use of different reporter-quencher dye pairs
• Compatible with most common commercially available fluorophores

EQ1 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence  resonance energy transfer (FRET) applications.

It can be used to quench reporter dyes: FAM, Cy2, TET, JOE, VIC, HEX, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Quasar^® 570, CAL Fluor^® Gold 540, CAL Fluor^® Orange 560, CAL Fluor^® Red 590, CAL Fluor^® Red 610, Alexa Fluor^® 430, Alexa Fluor^® 488, Alexa Fluor^® 532, Alexa Fluor^® 546, Alexa Fluor^® 555, Alexa Fluor^® 568, Alexa Fluor^® 594, DyLight^® 488, DyLight^® 550, DyLight^® 594, ATTO 488, ATTO 520, ATTO 532, ATTO Rho6G, ATTO 550, ATTO 565, ATTO Rho11, ATTO 590, ATTO 594.

The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.