EQ0 NHS ester

• Commonly used in molecular biology FRET applications to quench fluorophores
• NHS ester quencher dye can be conjugated to amine-labeled probes
• Enable multiplexing of probes with the use of different reporter-quencher dye pairs
• Compatible with most common commercially available fluorophores

EQ0 NHS ester is an amine-reactive quencher dye. It has a broad visible absorption but no detectable fluorescence emission, making it useful as an acceptor in fluorescence  resonance energy transfer (FRET) applications.

It can be used to quench reporter dyes: AMCA, AMCA-X, DEAC, DEAC-X, MCA, MCC, CF™ 350, CF™405S, CF™405M, Pacific Blue™, Cascade Blue^®, Marina Blue^®, Alexa Fluor^® 350, Alexa Fluor^® 405, DyLight^® 350, DyLight^® 405, ATTO 390, ATTO 425, ATTO 465, iFluor™350, iFLuor™405, BD Horizon V450, BUV395, eFluor^®450.

The EQ dyes are a family of non-fluorescent dyes that are designed for fluorescence quenching in FRET applications.