AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
This antibody is covered by our Worry-Free Guarantee.
Product Details
| Alternative Name |
Epidermal growth factor receptor, EGFR, HER-1 |
|---|---|
| Application |
ELISA, ICC, IP, Luminex, WB |
| Clone |
13G8 |
| Formulation |
Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Host |
Mouse |
| Immunogen |
Synthetic peptide corresponding to aa 1165-1186 of EGF receptor (epidermal growth factor receptor) conjugated to KLH. |
| Isotype |
IgG1 |
| Positive Control |
Included. (Prod. No. ALX-840-030) |
| Purity Detail |
Thiophilic adsorption and size exclusion chromatography purified. |
| Recommendation Dilutions/Conditions |
Immunocytochemistry (0.1-1µg/ml)Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Reconstitution |
Reconstitute with 1ml water (15 minutes at room temperature). |
| Species Reactivity |
Human, Mouse |
| Specificity |
Recognizes the C-terminus of the EGF receptor (aa 1165-1186). Recognition is independent of phosphorylation of Tyr1173. |
| UniProt ID |
P00533 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Use/Stability |
Stable at -80°C up to 1 year, at 4°C up to 3 months. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Mutated axon guidance gene PLXNB2 sustains growth and invasiveness of stem cells isolated from cancers of unknown primary: S. Brundu, et al.; EMBO Mol. Med. 15, e16104 (2023), Abstract
- Autocrine Signaling of NRP1 Ligand Galectin-1 Elicits Resistance to BRAF-Targeted Therapy in Melanoma Cells.: Corso, S., Giordano, S., et al.; Cancers (Basel) 12, (2020), Reactant(s): Human, Abstract
- Neuropilin-1 upregulation elicits adaptive resistance to oncogene-targeted therapies.: Rizzolio, S., Tamagnone, L., et al.; J. Clin. Invest. 128, 3976 (2018), Application(s): WB, Abstract
- Radiologic and Genomic Evolution of Individual Metastases during HER2 Blockade in Colorectal Cancer.: Siravegna, G., Lazzari, L., et al.; Cancer Cell 34, 148 (2018), Reactant(s): Human, Abstract
- Downregulating Neuropilin-2 Triggers a Novel Mechanism Enabling EGFR-Dependent Resistance to Oncogene-Targeted Therapies: Rizzolio, S., Battistini, C., et al.; Cancer Res. 78, 1058 (2018), Abstract
- MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation: S. Yoon, et al.; Immunity 47, 51 (2017), Abstract
- Vertical suppression of the EGFR pathway prevents onset of resistance in colorectal cancers: S. Misale, et al.; Nat. Commun. 6, 8305 (2015), Application(s): Immunoblot Analysis, Abstract — Full Text
- RAF Suppression Synergizes with MEK Inhibition in KRAS Mutant Cancer Cells: S. Lamba, et al.; Cell Rep. 8, 1475 (2014), Application(s): Western Blotting, Abstract — Full Text
- Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer: S. Misale, et al.; Nature 486, 532 (2012), Abstract
- Y1068 phosphorylation is the most sensitive target of disulfonated tetraphenylporphyrin-based photodynamic therapy on epidermal growth factor receptor: A. Weyergang, et al.; Biochem. Pharmacol. 74, 226 (2007), Application(s): Western blot, Abstract
- Abi-1 forms an epidermal growth factor-inducible complex with Cbl: role in receptor endocytosis: Tanos, B. E., Pendergast, A. M., et al.; Cell. Signal. 19, 1602 (2007), Abstract
- Abl tyrosine kinase regulates endocytosis of the epidermal growth factor receptor: Tanos, B., Pendergast, A. M., et al.; J. Biol. Chem. 281, 32714 (2006), Abstract
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form