DUB-Resistant K48-Linked Di-Ubiquitin

DUB-resistant, K48-linked Di-ubiquitin (Ub2) contains an amino acid substitution near the C-terminus of the distal ubiquitin that renders it resistant to deubiquitylases (DUBs). DUB-resistant ubiquitin chains are ideal reagents for structural or binding studies with deubiquitylases or other ubiquitin binding proteins. They can also be used as inhibitors or to determine the linkage-specificity of DUBs. The product contains a K48R mutation in the distal ubiquitin and a wild-type C-terminus on the proximal ubiquitin.