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DnaK (E. coli) monoclonal antibody (8E2/2)

ENZ-ABS680

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DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.

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This antibody is covered by our Worry-Free Guarantee.

DnaK (E. coli) monoclonal antibody (8E2/2) Western Blot — Western Blot analysis of ENZ-ABS680: Lane 1: MW Marker, Lane 2: DnaK (E. coli) recombinant protein (Prod. No. ADI-SPP-630), Lane 3: E. coli cell lysate.

Product Details

Alternative Name	Hsp70
Application	WB
Application Notes	Detects a band of ~70kDa by Western blot.
Clone	8E2/2
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Host	Mouse
Immunogen	E. coli DnaK.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:10,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from hybridoma tissue culture supernatant.
Species Reactivity	E. coli
UniProt ID	P0A6Y8 (strain K12)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Genetic Programming by Nitric Oxide-Sensing Gene Switch System in Tumor-Targeting Bacteria: Y. Qin, et al.; Biosensors 13, 266 (2023), Abstract
Omptin Proteases of Enterobacterales Show Conserved Regulation by the PhoPQ Two-Component System but Exhibit Divergent Protection from Antimicrobial Host Peptides and Complement: Y.H. Cho, et al.; Infect. Immun. 91, e00518 (2023), Abstract
Transcriptional insight into the effect of benzalkonium chloride on resistance and virulence potential in Salmonella Typhimurium: S. Park & H. Yoon; Microbiol. Res. 266, 127240 (2023), Abstract
Development of antisense peptide-peptide nucleic acids against fluoroquinolone-resistant Escherichia coli: S.K. Kim, et al.; J. Antimicrob. Chemother. 78, 2052 (2023), Abstract
Spatiotemporal regulation of the BarA/UvrY two-component signaling system: F.U. Contreras, et al.; J. Biol. Chem. 299, 104835 (2023), Abstract
The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches: A. Hipólito, et al.; Nucleic Acids Res. 50, 8566 (2022), Abstract
OmpR and Prc contribute to switch the Salmonella morphogenetic program in response to phagosome cues: D.L. Escarpa, et al.; Mol. Microbiol. 118, 477 (2022), Abstract

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