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DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
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Western blot analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein (Prod. No. ADI-SPP-630), Lane 3: E. coli cell lysate.
Expression of S. Tm ColIb is induced by iron limitation and the SOS response.(A) Organization of the ColIb locus showing location of the Fur and LexA repressor binding sites in the ColIb (cib) promoter and the immunity protein gene (imm). Overnight cultures of S. TmΔoriT (B) and S. TmΔoriT avir (C) carrying the reporter plasmid pcib-luc were re-inoculated 1∶20 in fresh LB with indicated supplements (0.25 µg/ml mitomycin C; 100 mM DTPA) and grown under aeration for 4 h. Cultures were normalized to OD600, bacteria were harvested and luciferase-activity was measured in bacterial lysates. Relative luminescence units (RLU) per unit OD600 are indicated. (D) Overnight cultures of indicated S. Tm strains were re-inoculated 1∶20 in fresh LB with indicated supplements (0.25 µg/ml mitomycin C; 100 mM DTPA) and grown under aeration for 4 h. OD600 of the cultures was normalized, bacteria were harvested and ColIb was detected in bacterial lysates as well as in the culture supernatant by immunoblot using an affinity-purified rabbit-α-ColIb antiserum. S. Tm DnaK was detected as loading control.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: Inflammation fuels colicin Ib-dependent competition of Salmonella serovar Typhimurium and E. coli in enterobacterial blooms. PLoS Pathog (2014)
| Regulatory Status |
RUO – Research Use Only |
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Related Products
| Application | ELISA, IHC, WB |
|---|---|
| Host | Goat |
| Species Reactivity | Mouse |
| Alternative Name | Heat shock protein 70, HSP70 |
|---|---|
| Purity | ≥95% (SDS-PAGE; Western blot) |
| Source | Produced in E. coli. |
| Alternative Name | Hsp70 |
|---|---|
| Application | WB |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity | E. coli |