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DnaJ (E. coli) polyclonal antibody

ADI-SPA-410

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DnaJ is a basic 41 kDa E. coli Heat shock protein which belongs to the molecular chaperone class of proteins. Bacterial DnaJ protein acts synergistically with the bacterial chaperone DnaK (Hsp70 homologue) and the other co-chaperone GrpE in suppressing eukaryotic and prokaryotic polypeptide aggregation, facilitating protein translocation through intracellular compartments or protein secretion, reactivating some partially aggregated enzymes, and activating pre-priming complex during initiation of DNA replication.

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This antibody is covered by our Worry-Free Guarantee.

DnaJ (E. coli) polyclonal antibody Western blot — Western blot analysis: Lane 1: DnaJ Recombinant E. coli Protein.

Product Details

Alternative Name	HSP40, Heat shock protein 40, Heat shock protein J
Application	IP, WB
Application Notes	Detects a band of ~41kDa by Western blot.
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Host	Rabbit
Immunogen	E. coli DnaJ.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Immunoprecipitation (1:200)Western Blot (1:1,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	E. coli
UniProt ID	P08622
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

RNA-dependent proteome solubility maintenance in Escherichia coli lysates analysed by quantitative mass spectrometry: Proteomic characterization in terms of isoelectric point, structural disorder, functional hub, and chaperone network.: Park, C., Han, B., et al.; RNA Biol. 21, 1 (2024), Application(s): WB / Reactant(s): E. coli, Abstract
Selection of a de novo gene that can promote survival of Escherichia coli by modulating protein homeostasis pathways: I. Frumkin & M.T. Laub; Nat. Ecol. Evol. 7, 2067 (2023), Application(s): WB, Abstract
The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli.: Valdez-Cruz, N. A., Perez, N. O., et al.; Appl. Microbiol. Biotechnol. 106, 2883 (2022), Application(s): WB / Reactant(s): E. coli, Abstract
Chaperone activity of serine protease HtrA of Helicobacter pylori as a crucial survival factor under stress conditions: Zarzecka, U., Harrer, A., et al.; Cell Commun. Signal. 17, 161 (2019), Abstract
Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis: Khodaparast, L., Khodaparast, L., et al.; Nat. Commun. 9, 866 (2018), Abstract
Overproduction of bacterial protein disulfide isomerase (DsbC) and its modulator (DsbD) markedly enhances periplasmic production of human nerve growth factor in Escherichia coli: T. Yura, et al.; J. Biol. Chem. 276, 14393 (2001), Application(s): WB using bacteria (E. coli) samples, Abstract
Hsp70 and hsp40 chaperones can inhibit self-assembly of polyglutamine proteins into amyloid-like fibrils: F.U. Hartl, et al.; PNAS 97, 7841 (2000), Application(s): WB using bacteria (E. coli) samples, Abstract

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DnaJ (E. coli) polyclonal antibody

ADI-SPA-410

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

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