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Spin trapping reagent
Cell permeable hydrophilic spin trap for both in vivo and in vitro studies of superoxide, O-, C-, S- and N- centered free radicals.
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Product Details
| Alternative Name |
5,5-Dimethyl-1-pyrroline-N-oxide, 2,2-Dimethyl-3,4-dihydro-2H-pyrrole 1-oxide |
|---|---|
| Appearance |
Pale yellow solid or oil. |
| CAS |
3317-61-1 |
| Formula |
C6H11NO |
| MW |
113.2 |
| Purity |
>98% (HPLC) |
| Solubility |
Soluble in DMSO (up to 25 mg/ml) or Ethanol (up to 25 mg/ml). |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. |
|---|---|
| Handling |
Protect from light and moisture. Keep under inert gas. Hygroscopic. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Free Radical Inhibition Using a Water-Soluble Curcumin Complex, NDS27: Mechanism Study Using EPR, Chemiluminescence, and Docking: A.M. Mickalad, et al.; Antioxidants 13, 80 (2024), Abstract
- Investigation of novel radical scavenging mechanisms in the alkaline environment: Green, sustainable and environmentally friendly antioxidative agent(s): M. Antonijević, et al.; Sci. Total Environ. 912, 169307 (2024), Abstract
- Thioxanthone-Based Siloxane Photosensitizer for Cationic/Radical Photopolymerization and Photoinduced Sol-Gel Reactions: T.T.T. Nguyen, et al.; Molecules 29, 255 (2024), Abstract
- Oxidation 1-methyl naphthalene based on the synergy of environmentally persistent free radicals (EPFRs) and PAHs in particulate matter (PM) surface: A. Ghimire, et al.; Chemosphere 341, 140002 (2023), Abstract
- Dynamin-related protein 1 regulates substrate oxidation in skeletal muscle by stabilizing cellular and mitochondrial calcium dynamics: W.T. King, et al.; J. Biol. Chem. 297, 101196 (2021), Abstract
- Adsorption of Arsenic Ions Transforms Surface Reactivity of Engineered Cerium Oxide Nanoparticles: X. Bi, et al.; Environ. Sci. Technol. 54, 9437 (2020), Abstract — Full Text
- Using MRI to measure in vivo free radical production and perfusion dynamics in a mouse model of elevated oxidative stress and neurogenic atrophy: Ahn, B., Smith, N., et al.; Redox Biol. 26, 101308 (2019), Abstract
- Expansive Vascular Remodeling and Increased Vascular Calcification Response to Cholecalciferol in a Murine Model of Obesity and Insulin Resistance: Carmo, L. S., Burdmann, E. A., et al.; Arterioscler. Thromb. Vasc. Biol. 39, 200 (2019), Abstract
- Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development: M. Takamiya, et al.; Sci. Rep. 6, 25046 (2016), Application(s): Immuno spin trapping assay with DMPO, Abstract — Full Text
- Control of grazing by light availability via light-dependent, wound-induced metabolites: The role of reactive oxygen species: R.E. McDowell, et al.; J. Exp. Mar. Bio. Ecol. 477, 86 (2016), Application(s): Electron paramagnetic resonance
- Radicalization of Glyceraldehyde-3-Phosphate Dehydrogenase by HOCl in Living Cells: S.E. Gomez-Mejiba; Enz. Eng. 4, 134 (2015), Application(s): Cell culture, Full Text
- OKN-007 decreases free radical levels in a preclinical F98 rat glioma model: P. Coutinho de Souza, et al.; Free Radic. Biol. Med. 87, 157 (2015), Abstract — Full Text
- Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces Cerevisiae by photo-Fenton at near-neutral pH: S. Giannakis, et al.; Appl. Catal. B 185, 150 (2015), Application(s): Cell culture
- Different mechanisms are involved in oxidative DNA damage and genotoxicity induction by ZnO and TiO2 nanoparticles in human colon carcinoma cells: A. Zijno, et al.; Toxicol. In Vitro 29, 1503 (2015), Application(s): EPR measurements, Abstract
- Cavitation during the protein misfolding cyclic amplification (PMCA) method – the trigger for de novo prion generation: C.L. Haigh & S.C. Drew; Biochem. Biophys. Res. Commun. 461, 494 (2015), Application(s): Cell Culture, Abstract
- Direct radical scavenging activity of benzbromarone provides beneficial antioxidant properties for hyperuricemia treatment: D. Kadowaki, et al.; Biol. Pharm. Bull. 38, 487 (2015), Application(s): Electron Paramagnetic Resonance Spectroscopy, Abstract — Full Text
- The cytotoxicity of the anticancer drug elesclomol is due to oxidative stress indirectly mediated through its complex with Cu(II): B.B. Hasinoff, et al.; J. Inorg. Biochem. 137, 22 (2014), Abstract
- Thymosin β4-sulfoxide attenuates inflammatory cell infiltration and promotes cardiac wound healing: M.A. Evans, et al.; Nat. Commun. 4, 2081 (2013), Application(s): Free-Radical Spin Trap Analysis, Abstract — Full Text
- Human Indoleamine 2,3-Dioxygenase Is a Catalyst of Physiological Heme Peroxidase Reactions: M. Freewan, et al.; J. Biol. Chem. 288, 1548 (2013), Application(s): Immuno-Spin Trapping coupled with Western Blotting, Abstract — Full Text
- Tar Balls from Deep Water Horizon Oil Spill: Environmentally Persistent Free Radicals (EPFR) Formation During Crude Weathering: L.W. Kiruri, et al.; Environ. Sci. Technol. 47, 4220 (2013), Application(s): Electron paramagnetic resonance (EPR) spectroscopy, Abstract — Full Text
- Hydrogen peroxide induce modifications of human extracellular superoxide dismutase that results in enzyme inhibition: R.H.Gottfredsen, et al.; Redox Biol. 1, 24 (2013), Abstract — Full Text
- Red Ginseng Extract Attenuates Kainate-Induced Excitotoxicity by Antioxidative Effects: J. Y Han, et al.; Evid. Based Complement. Alternat. Med. 2012, 479016 (2012), Application(s): Electron spin resonance (ESR) spectroscopy, Abstract — Full Text
- Strawberry Polyphenols Attenuate Ethanol-Induced Gastric Lesions in Rats by Activation of Antioxidant Enzymes and Attenuation of MDA Increase: J.M. Alvarez-Suarez, et al.; PLoS One 6, e25878 (2011), Application(s): Electron paramagnetic resonance (EPR) spectroscopy, Abstract — Full Text
- Environmentally Persistent Free Radicals (EPFRs). 2. Are Free Hydroxyl Radicals Generated in Aqueous Solutions?: L. Khachatryan, et al.; Environ. Sci. Technol. 45, 9232 (2011), Application(s): Electron paramagnetic resonance (EPR) spectroscopy, Abstract — Full Text
- Environmentally Persistent Free Radicals (EPFRs). 1. Generation of Reactive Oxygen Species in Aqueous Solutions: L. Khachatryan, et al.; Environ. Sci. Technol. 45, 8559 (2011), Application(s): Electron paramagnetic resonance (EPR) spectroscopy, Abstract — Full Text
- Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation: Y. Feng, et al.; PLoS Biol. 8, e1000562 (2010), Application(s): Immuno-Spin Trapping, Abstract — Full Text
- Immuno-spin trapping of protein and DNA radicals: tagging” free radicals to locate and understand the redox process”””: S.E. Gomez-Mejiba, et al.; Free Radic. Biol. Med. 46, 853 (2009), Abstract
- Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice: S. Chatterjee, et al.; Free Radic. Biol. Med. 46, 454 (2009), Abstract
- Immunolocalization of hypochlorite-induced, catalase-bound free radical formation in mouse hepatocytes: M.G. Bonini et al.; Free Radic. Biol. Med. 42, 530 (2007), Abstract
- Nitric oxide decreases the stability of DMPO spin adducts: K.J. Reszka, et al.; Nitric Oxide 15, 133 (2006), Abstract
- Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species: H. Shi, et al.; Arch. Biochem. Biophys. 437, 59 (2005), Abstract
- Superoxide radical anion adduct of 5,5-dimethyl-1-pyrroline N-oxide (DMPO). 2. The thermodynamics of decay and EPR spectral properties: F.A. Villamena, et al.; J. Phys. Chem. A. Mol. Spectrosc. Kinet. Environ. Gen. Theory 109, 6089 (2005), Abstract
- Characterization of the high-resolution ESR spectra of superoxide radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Analysis of conformational exchange: S. Dikalov, et al.; Free Radic. Res. 39, 825 (2005), Abstract
- Superoxide radical anion adduct of 5,5-dimethyl-1-pyrroline n-oxide (DMPO). 1. The thermodynamics of formation and its acidity: F.A. Villamena, et al.; J. Phys. Chem. A. Mol. Spectrosc. Kinet. Environ. Gen. Theory 109, 6083 (2005), Abstract
- Assignment of the EPR spectrum of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) superoxide spin adduct: J.L. Clement, et al.; J. Org. Chem. 70, 1198 (2005), Abstract
- Hydroxyl radical generation caused by the reaction of singlet oxygen with a spin trap, DMPO, increases significantly in the presence of biological reductants: C. Nishizawa, et al.; Free Radic. Res. 38, 385 (2004), Abstract
- A new kinetic approach to the evaluation of rate constants for the spin trapping of superoxide/hydroperoxyl radical by nitrones in aqueous media: R. Lauricella, et al.; Org. Biomol. Chem. 2, 1304 (2004), Abstract
- ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes: K. Anzai, et al.; Arch. Biochem. Biophys. 415, 251 (2003), Abstract
- Generation of nitric oxide from spin-trapping agents under oxidative conditions: K. Saito, et al.; Biosci. Biotech. Biochem. 62, 275 (1998)
- Formation of spin trap adducts during the decomposition of peroxynitrite: R.M. Gatti, et al.; Arch. Biochem. Biophys. 349, 36 (1998), Abstract
- Formation of reactive oxygen species by pentaerithrityltetranitrate and glyceryl trinitrate in vitro and development of nitrate tolerance: S. Dikalov, et al.; J. Pharmacol. Exp. Ther. 286, 938 (1998), Abstract — Full Text
- Superoxide generation from endothelial nitric-oxide synthase. A Ca2+/calmodulin-dependent and tetrahydrobiopterin regulatory process: Y. Xia, et al.; J. Biol. Chem. 273, 25804 (1998), Abstract — Full Text
- Electron spin resonance detection of extracellular superoxide anion released by cultured endothelial cells: J.P. Souchard, et al.; Free Radic. Res. 29, 441 (1998), Abstract
- MDL 101,002, a free radical spin trap, is efficacious in permanent and transient focal ischemia models: M.P. Johnson, et al.; Life Sci. 63, 241 (1998), Abstract
- Oxygen-centered spin adducts of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and 2H-imidazole 1-oxides: A.G. Krainev, et al.; J. Magn. Reson. B. 111, 272 (1996), Abstract
- Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis: A.F. Slater, et al.; Biochem. J. 306, 771 (1995), Abstract
- Nitric oxide, superoxide and peroxynitrite: putative mediators of NMDA-induced cell death in cerebellar granule cells: M. Lafon-Cazal, et al.; Neuropharmacology 32, 1259 (1993), Abstract
- The spin trapping of superoxide and hydroxyl free radicals with DMPO (5,5-dimethylpyrroline-N-oxide): more about iron: G.R. Buettner; Free Radic. Res. Comm. 19 Suppl 1, S79 (1993), Abstract
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